基于高通量测序的木枣鲜食枣果成熟过程转录组分析

Transcriptome Data Analysis of Fresh Jujube Fruits During Ripening Process Based on High-throughput Sequencing

为了研究木枣枣果成熟时由青果向红果转变的分子机理,本研究使用Illumina高通量测序平台对木枣和其衍生种大木枣白熟期青果和全红期红果进行转录组测序分析。经过测序共得到Clean Data 80.12 Gb,各样品Clean Data平均为6.68 Gb,GC含量为45.00%,Q 30>93.63%,与参考基因组的比对效率在79.97%~86.44%之间,共发掘1631个新基因。样品间共筛选出4905个差异表达基因,其中有8个差异表达基因是所有样品组合共有的。全红期红果与白熟期青果(R vs.G)组合注释到各数据库的DEG基因数(3278)明显多于其他3个组合,木枣与大木枣白熟期青果(MG vs.DG)间的DEG基因数(1285)多于全红期红果(MR vs.DR)间的DEG基因数(857)。枣果在白熟期和红果期发育阶段的差异表达基因参与了细胞发育、代谢过程及单一生物学过程,并具有较好的结合能力和催化活性,主要参与α-亚麻酸代谢,半乳糖代谢,光合作用-天线蛋白质等代谢途径。同时,还预测检测到2982个转录调控因子,归于转录因子(TF,1298)、转录调节子(TR,368)、蛋白激酶(PK,1316)3大类,分别以AP2-EREBP家族(112)、m TERF家族(46)、RLK-Pelle家族(1050)的基因最多。该转录组测序分析结果为寻找枣果由白熟期向全红期转变的相关基因提供理论参考。

In order to study the molecular mechanism of the transformation from green to red when the jujube fruit matures,transcriptome analysis on the green fruits in the white mature period and the red fruits in the full red period of mu jujube and its derived cultivar Damuzao were used by Illumina Hiseq 4000 sequencing in this study.About 80.12 Gb Clean Data in total were obtained,each sample reached 6.68 Gb,the GC content was 45.00%,Q 30>93.63%,the mapped reads efficiency was between 79.97%and 86.44%,and about 1631 new genes were discovered.There were 4905 differentially expressed genes(DEGs)in transcriptome data at different samples,of which 8 DEGs were shared by all sample groups.A total of 3278 DEGs in the group of the red fruits in the full red period and the green fruits in the white mature period(R vs.G)annotated in each database,it was significantly more than the other three groups.The DEGs number of green fruits between Muzao and Damuzao in the white mature period(MG vs.DG)was up to 1285,it was higher than the number of red fruits(857)in the full red period(MR vs.DR).The differentially expressed genes in all sample groups were involved in cell development,metabolic process and single-organism process,and had good ability of binding and catalytic activity,mainly significantly enriched in the metabolic pathways on alpha-linolenic acid metabolism,galactose metabolism,photosynthesis-antenna proteins.Meanwhile,2982 transcriptional regulators were predicted in 4905 DEGs,which belonged to three types of transcription factor(TF,1298),transcriptional regulator(TR,368),and protein kinase(PK,1316).The number of AP2-EREBP family,mTERF family and RLK-Pelle family in each type were the most,with 112,46,1050,respectively.This transcriptome sequencing analysis could provide certain theoretical reference for the study of finding the key genes in jujube fruit from the period of white mature to full red.