细菌纳米纤维素/聚乙烯醇小径人工血管的制备及其表征

Preparation and Characterization of BNC/PVASmall-diameter Artificial Blood Vessels

细菌纳米纤维素(bacterial nano-cellulose,BNC)有着良好的生物相容性和力学性能,已被广泛研究用于人工血管等生物医学领域。当纯的BNC凝胶管用于小口径人工血管时,缝合强度等力学性能以及长期抗凝血性等方面不足。为改善以上性能,本研究首次采用压力注射聚乙烯醇(polyvinyl alcohol,PVA)与相分离技术相结合的方法制备BNC/PVA复合管,同时对复合管进行了理化性能与生物相容性表征。结果表明BNC/PVA复合管的轴向拉伸性能、缝合强度、抗渗漏性、爆破压指标均大于BNC管。复合管减少了血小板的粘附,延长了血浆复钙时间,抗凝性优于BNC管｡BNC/PVA复合管对于HUVECs生长增殖无毒害作用。以15%PVA初始浓度复合的BNC/PVA15%管具有良好的力学性能、血液相容性和细胞相容性,表明该复合管在小口径人口血管领域具有良好的应用前景。

Bacterial nano-cellulose(BNC)has been widely used in biomedical research such as artificial blood vessels due to its good biocompatibility and mechanical properties.When pure BNC gel tubes are used in small-caliber artificial blood vessels,there are still deficiencies in suture strength and long-term anticoagulant properties.Further study is therefore required.In the current study to obtain better mechanical strength,the preparation of BNC/PVA composite tubes was performed for the first time by using pressure injection of polyvinyl alcohol(PVA)followed by phase separation technology.The results showed that the axial tensile property,suture strength,leakage resistance and burst pressure index of the BNC/PVA composite tubes were all higher than BNC tube.In comparison to BNC tube,the composite tubes reduced platelet adhesion,prolonged the time of plasma calcium recovery,and had better anticoagulant property.BNC/PVA composite tubes had no toxic effect on growth and proliferation of HUVECs.The BNC/PVA15%tube made with PVA at initial concentration of 15%had good mechanical properties,blood compatibility and cell compatibility,suggesting a good prospect in the application of small-caliber artificial blood vessels.