一种高抗性产海藻糖酿酒酵母菌株的开发

Development of a high resistant strain of Saccharomyces cerevisiae producing trehalose

高抗性产海藻糖酿酒酵母菌株ZGJ-4 (专利号ZL201710503931.7)是项目组从自然界分离纯化且育种而得,菌种保藏号为CCTCC NO:M2012473。试验从菌学特征、抗性研究、酵母菌ZGJ-4发酵制备海藻糖的工艺研究等三方面对ZGJ-4的开发价值进行了探讨。结果表明,酵母菌ZGJ-4的发酵能力强,当高浓度发酵结束后,让菌体饥饿处理3 h可得到含海藻糖高的产物,经HPLC检测每100 g干重细胞含有35 g海藻糖,海藻糖得率>80%。由于ZGJ-4具备抗干旱、耐酸、抗高温等特性,所产海藻糖制备率高,可在食品、食品保鲜、医药等领域应用,具有良好的开发利用潜力。

The highly resistant trehalose producing yeast strain ZGJ-4(patent NO.ZL201710503931.7)was isolated and purified from the nature and bred by the project team.The strain conservation number is CCTCC NO:M 2012473.In the experiment,the development value of ZGJ-4 was discussed from three aspects:bacteriological characteristics,resistance research,and fermentation technology of yeast ZGJ-4 to prepare trehalose.The results showed that the project team possessed a strong fermentation capacity of yeast ZGJ-4.After the end of high concentration fermentation,the bacteria were starved for 3 hours to obtain products with high trehalose content.The trehalose yield was>80%,with 35 grams of trehalose per 100 grams of dry weight cells detected by HPLC.ZGJ-4 has the characteristics of drought resistance,acid resistance and high temperature resistance,high preparation rate of trehalose produced,and can be applied in food,food preservation,medicine and other fields,with good development and utilization potential.