摘要
根据科研成果,设计了甲醛修饰聚乙烯亚胺包覆纳米金的荧光探针合成及其荧光量子效率测试的综合实验。以聚乙烯亚胺、甲醛、四氯金酸为原料,在90℃下水浴回流1 h得到甲醛修饰聚乙烯亚胺包覆的纳米金荧光探针材料。以硫酸奎宁作为参比,通过"比较法"测试该荧光材料的相对量子产率并与"一点法"进行比较。通过本实验项目的开展,希望学生能掌握紫外可见分光光度计、荧光分光光度计及红外分光光度计几种光谱类仪器的结构及原理,掌握这些设备的基本操作技能及应用,掌握荧光量子效率的测试原理和测试方法,提高分析问题和解决实际问题的能力。
According to some research achievements, a comprehensive experiment containing the synthesis of formaldehyde modified polyethyleneimine and determination of fluorescence quantum yield was designed. The formaldehyde modified polyethyleneimine was obtained by using polyethyleneimine and formaldehyde as raw materials and refluxing at 90℃ for 1 hour in a water bath. Using quinine sulfate as a reference, the relative quantum yield of the fluorescent material was tested by the "comparative method" and compared with the "one-point method". Through the development of this experimental project, students are expected to master the structure and principles of several spectroscopic instruments such as ultraviolet-visible spectrophotometer, fluorescence spectrophotometer and infrared spectrophotometer, master the basic operation skills and applications of these devices, and master the fluorescence quantum efficiency. Test principles and test methods to improve students’ ability to analyze problems and solve practical problems.
作者
王娜
杨洪
王良臣
熊婷婷
WANG Na;YANG Hong;WANG Liangchen;XIONG Tingting(School of Chemistry and Chemical Engineering,Southwest Petroleum University,Chengdu 610500,China)
出处
《实验科学与技术》
2020年第3期109-113,139,共6页
Experiment Science and Technology
基金
西南石油大学科研启航计划项目(2015QHZ016)
国家大学生创新创业训练项目(201810615030)。
关键词
仪器分析
聚乙烯亚胺
甲醛
修饰
荧光量子产率
instrumental analysis
polyethyleneimine
formaldehyde
modified
fluorescence quantum efficiency
