miR-96与miR-103在肝癌组织中的表达及其与预后的关系

Expressions of miR-96 and miR-103 in Hepatocellular Carcinoma and Their Correlation with Prognosis

目的探讨miR-96与miR-103在肝癌组织中的表达及其与预后的关系。方法选取122例肝细胞癌患者作为本研究对象。聚合酶链式反应检测miR-96和miR-103表达水平,采用单因素和多因素Cox回归模型,探讨肝癌患者中位生存时间的危险因素并建立预测模型。结果肝癌组织中miR-96和miR-103相对表达水平均显著高于癌旁正常组织;肝癌组织中miR-96和miR-103的表达与肿瘤直径、淋巴结转移、临床分期及分化程度均有关(均P<0.05),而与性别、年龄及合并肝硬化等无关(P>0.05);所有患者随访60月,中位生存时间为37.8月。单因素分析结果显示:肝细胞癌患者中位生存时间与肿瘤直径、淋巴结转移、TNM分期、病理分化程度、miR-96和miR-103表达水平差异均有统计学意义(均P<0.05)。多因素分析结果显示:TNM分期为Ⅲ~Ⅳ、低分化、淋巴结转移、miR-96和miR-103高表达是肝癌患者中位生存时间独立危险因素。结论肝癌组织中miR-96和miR-103的高表达是患者预后差的独立影响因素,可作为原发性肝癌患者预后评估指标。

Objective To explore the expressions of miR-96 and miR-103 in hepatocellular carcinoma tissues and their relation with prognosis.Methods We selected 122 patients with hepatocellular carcinoma as the observation subjects.The expression levels of miR-96 and miR-103 were detected,and univariate and multivariate Cox regression models were used to analyze the risk factors of median survival time of hepatocellular carcinoma patients and to establish a predictive model.Results The relative expression levels of miR-96 and miR-103 in hepatocellular carcinoma tissues were significantly higher than those in paracancerous tissues;the expression of miR-96 and miR-103 in HCC were correlated with tumor diameter,lymph node metastasis,clinical stage and degree of differentiation(all P<0.05).All cases were followed up for 60 months,and the median survival time was 37.8 months.The expression of miR-96 and miR-103 in HCC were correlated with tumor diameter,lymph node metastasis,clinical stage and differentiation,but not with sex,age or liver cirrhosis.Multivariate analysis showed that TNM stageⅢ-Ⅳ,poor differentiation,lymph node metastasis,high expression of miR-96 and miR-103 were independent risk factors for median survival time of patients with primary hepatic carcinoma.Conclusion The high expression of miR-96 and miR-103 in liver cancer tissues are the independent influence factors of poor prognosis in primary hepatic carcinoma patients and could be used as the indexes of prognostic evaluation.