清肝降浊颗粒对糖尿病脂肪肝大鼠脂代谢相关炎症因子与血管内皮功能的影响

Effect of Qinggan Jiangzhuo Granules on the Change of Inflammatory Cytokinesin and Endothelial Function in Nonalcoholic Fatty Liver of Type 2 Diabetic Mellitus Rats

目的探讨清肝降浊颗粒对2型糖尿病(T2DM)合并非酒精性脂肪肝病(NAFLD)大鼠炎症因子与内皮功能的影响。方法链脲佐菌素(STZ)与高脂饮食结合建立T2DM合并NAFLD大鼠模型。造模成功后,将模型大鼠随机分成模型对照组;血脂康胶囊400 mg/kg组;清肝降浊颗粒设800、400和200 mg/kg 3个给药剂量组,每组各10只。另设10只大鼠正常对照组。给药8周后,检测与2型糖尿病合并非酒精性脂肪肝病大鼠发病相关的各项指标:为血清中FBG、TC、TG、LDL-cho、FFAs、hs-CRP、TNF-α、IL-6 和ET-1浓度。结果清肝降浊颗粒对糖尿病脂肪肝大鼠血清TC、TG 、LDL-cho和FFAs水平的异常升高均具有明显的降低作用,与模型组比较,分别为P<0.01或P<0.05;降低糖尿病脂肪肝大鼠血清hs-CRP、TNF-α、IL-6 和ET-1浓度,与模型组比较,分别为P<0.01或P<0.05。结论清肝降浊颗粒对2型糖尿病合并非酒精性脂肪肝大鼠的脂质代谢,炎症、血管内皮功能障碍具有显著的治疗作用。

Objective To explore whether Qinggan Jiangzhuo granules therapeutic effect on the change of endothelial function and inflammatory cytokinesin in nonalcoholic fatty liver of type 2 diabetic mellitus rats.Methods The model group were induced T2DM with NAFLD by streptozotocin(STZ)and high-fat diet.After successfully establishing the models,they were randomly divided into the normal group and the model group;the Xuezhikang 400 mg/kg dose group;800 mg/kg,400 mg/kg and 200 mg/kg dose groups of Qinggan Jiangzhuo granules of 10 each.8 weeks after the treatment,we detected the indicators closely related to nonalcoholic fatty liver of type 2 diabetic mellitus rats.The indicators include:blood biochemical indicator detection;Serum lipid parameters(FBG,TC,TG,LDL-cho,FFAs,hs-CRP,TNF-α,IL-6 and ET-1).Results Qinggan Jiangzhuo granules can obviously decrease abnormal increase of nonalcoholic fatty liver of type 2 diabetic mellitus rats serum TC,TG,LDL-cho and FFAs levels,compared with model group,respectively,P<0.01 or P<0.05.It also can decrease hs-CRP,TNF-α,IL-6 and ET-1 content,compared with model group,respectively,P<0.01 or P<0.05.Conclusion Qinggan Jiangzhuo granules has significant therapeutic effects on lipid metabolism,inflammation,vascularendo-thelial dysfunction in rats with type 2 diabetes mellitus and nonalcoholic fatty liver.