Rsf-1和α-SMA与c-erbB-2在胃癌中的表达及意义

Expression and significance of Rsf-1,α-SMA and c-erbB-2 in precancerous lesions of gastric cancer

目的探讨染色质重塑因子1(Rsf-1)、α平滑肌肌动蛋白(α-SMA)、c-erbB-2在胃癌中的表达及意义。方法收集2016年9月~2018年9月我院经过病理证实的胃癌标本30例,癌前病变组织标本30例,正常胃黏膜组织标本10例。免疫组化染色及Western Blot检测Rsf-1、α-SMA、c-erbB-2表达。结果胃癌组织Rsf-1、α-SMA、c-erbB-2阳性表达率高于胃癌前病变组织和正常胃黏膜组织(P<0.05)。胃癌黏膜、肌层、浆膜浸润深度Rsf-1、α-SMA、c-erbB-2阳性表达率比较差异无统计学意义(P>0.05)。有淋巴结转移胃癌Rsf-1、α-SMA、c-erbB-2阳性表达率高于无淋巴结转移胃癌(P<0.05)。Rsf-1、c-erbB-2、α-SMA之间呈正相关(P<0.05)。结论Rsf-1、α-SMA、c-erbB-2在胃癌中呈高表达,其表达水平与胃癌浸润深度以及淋巴结转移有关,在胃癌发展过程中具有重要作用。

Objective To study the expression and significance of chromatin remodeling factor 1(Rsf-1),αsmooth muscle actin(α-SMA)and c-erbB-2 in precancerous lesions of gastric cancer.Methods From September 2016 to September 2018,30 specimens of gastric cancer,30 specimens of precancerous lesions and 10 specimens of normal gastric mucosa were collected.The expression of Rsf-1,α-SMA and c-erbB-2 was detected by enzyme-linked immunosorbent assay(ELISA)after immunohistochemical staining.Results The positive rates of RSF-1,α-SMA and c erbB 2 in gastric cancer were higher than those in precancerous lesions and normal gastric mucosa(P<0.05).There was no significant difference in RSF-1,α-SMA,c-erbB-2 positive expression rates among gastric cancer mucosa,sarcolemma and serosa(P>0.05).The positive rates of RSF-1,α-SMA and c-erbB-2 in gastric cancer with lymph node metastasis were higher than those in gastric cancer without lymph node metastasis(P<0.05).There was a positive correlation between RSF-1,c-erbB-2 andα-SMA(P<0.05).Conclusion Rsf-1,alpha-SMA and c-erbB-2 were highly expressed in gastric cancer tissues,which were positively correlated with the depth of invasion and lymph node metastasis,and played an important role in the development of gastric cancer.