强直性脊柱炎患者HLA-B27表达情况及基因亚型检测分析

Expression and gene isoforms of HLA-B27 in ankylosing spondylitis patients

目的分析强直性脊柱炎(AS)患者HLA-B27阳性表达情况及基因亚型分布。方法抽取2018年1月至12月惠州市中心人民医院收治的47例AS患者为观察组,并以47例同期健康体检者作为对照组。采用流式细胞术检验两组HLA-B27表达情况,采用荧光定量聚合酶链式反应分析HLA-B27阳性者的基因亚型情况,并对两组检测结果进行分析。结果观察组HLA-B27阳性表达率(91.49%,43/47)高于对照组(8.51%,4/47),差异有统计学意义(P<0.05)。对照组及观察组HLA-B27基因阳性者均以男性为主,年龄集中于20~40岁,两组比较差异未见统计学意义(P>0.05)。对照组HLA-B27基因阳性者的基因亚型以HLA-B2706、HLA-B2709为主,观察组HLA-B27基因阳性者的基因亚型以HLA-B2704、HLA-B2705为主。观察组HLA-B2704、HLA-B2705基因亚型者多于对照组,HLA-B2706、HLA-B2709基因亚型者少于对照组,差异有统计学意义(P<0.05)。结论HLA-B27阳性常见于男性青壮年,AS患者多有HLA-B27阳性表达,且以HLA-B2704、HLA-B2705基因亚型为主,与健康体检者存在明显差异。临床中加强HLA-B27检测及基因分型分析有助于尽早诊断AS。

Objective To analyze the expression and gene isoforms of HLA-B27 in patients with ankylosing spondiylitis(AS).Methods Forty-seven AS patients treated in Central People’s Hospital of Huizhou from January to December of 2018 were selected as observation group,and 47 persons who underwent physical examination in the same period were selected as control group.The expression of HLA-B27 of the two groups were determined by flow cytometry,and gene isoforms of HLA-B27 in positive patients were detected by fluorescence quantitative polymerase chain reaction.The examination results of the two groups were analyzed.Results The positive rate of HLA-B27 of the observation group was 91.49%(43/47),higher than the 8.51%(4/47)of the control group(P<0.05).Most of patients with HLA-B27 positive in the two groups were males,aged 20 to 40 years,and the difference between the two groups was not significant(P>0.05).HLA-B2706 and HLA-B2709 were predominant types of gene isoform in the control group,while HLA-B2704 and HLA-B2705 were main gene isoforms in the observation group.The proportion of patients with HLA-B2704 or HLA-B2705 in the observation group was bigger than that in the control group,but the proportion of patients with HLA-B2706 and HLA-B2709 in the observation group was less(P<0.05).Conclusions HLA-B27 positive is common in young male adults.Moreover,most of AS patients are HLA-B27 positive,with HLA-B2704 or HLA-B2705 as predominant gene isoforms,which is significantly different from healthy persons.Performing determination on gene iosforms of HLA-B27 is conductive to early diagnosis and treatment of AS in clinical.