免疫比浊法检测肌酸激酶同工酶质量对急性心肌梗死的诊断价值

Value of detection of creatine kinase isoenzyme mass by immunoturbidimetry in the diagnosis of acute myocardial infarction

目的探讨免疫比浊法检测肌酸激酶同工酶(CKMB)质量对急性心肌梗死的诊断价值。方法将2018年6月至2019年6月晋城大医院收治的150例急性心肌梗死(AMI)患者作为观察组,选择同期150例健康体检者作为对照组,对比两组血清心肌肌钙蛋白T(cTnT)、肌红蛋白(MYO)、CKMB质量水平;用电化学发光法与免疫比浊法分别检测观察组患者的CKMB质量水平,对比两种检测方法的阳性检出率。结果观察组患者的血清cTnT、MYO、CKMB质量水平均高于对照组,差异有统计学意义(P<0.05)。观察组经电化学发光法检测的CKMB质量为(6.30±3.63)μg/L,经免疫比浊法检测的CKMB质量为(6.26±3.78)μg/L,两者比较差异未见统计学意义(P>0.05);电化学发光法与免疫比浊法检测CKMB质量阳性检出率[96.00%(144/150)比94.67%(142/150)]比较,差异未见统计学意义(P>0.05)。结论检测CKMB质量对诊断AMI具有重要意义,使用免疫比浊法法检测CKMB质量和电化学发光法检测结果无差异,更适合于基层医院的发展,而且具有较高的阳性检出率。

Objective To investigate the value of determination of creatine kinase isoenzyme(CKMB)mass by immunoturbidimetry in the diagnosis of acute myocardial infarction.Methods A total of 150 patients with acute myocardial infarction(AMI)admitted to Jincheng General Hospital from June 2018 to June 2019 were enrolled into the observation group.And 150 healthy persons who underwent physical examination in the same period were selected as the control group.Serum levels of cardiac troponin T(cTnT),myoglobin(MYO)and CKMB mass in the two groups were compared.CKMB mass levels in the observation group were measured by electrochemiluminescence and immunoturbidimetry.And the positive detection rates of the two methods were compared.Results The serum cTnT,MYO and CKMB mass levels in the observation group were significantly higher than those in the control group(P<0.05).In the observation group,the levels of CKMB mass were(6.30±3.63)μg/L detected by electrochemiluminescence and(6.26±3.78)μg/L detected by immunoturbidimetry,and the difference between the two methods was not statistically significant(P>0.05).There was no significant difference in positive rate of CKMB mass between electroluminescence(96.00%,144/150)and immunoturbidimetry(94.67%,142/150),P>0.05.Conclusions The detection of CKMB mass is of great significance for the diagnosis of AMI.The results of immunoturbidimetric method in detecting CKMB mass are not significantly different from the electrochemical luminescence,but the former is more suitable for the development of primary hospitals and has a high positive detection rate.