高羊茅FaRVE8基因的克隆、亚细胞定位及表达分析

Cloning,subcellular localization and expression analysis of the RVE 8 gene from Festuca arundinacea

生物钟是一个复杂的调控网络,在环境的不断变化中促进植物的生长,REVEILLE8(RVE8)是生物钟的重要基因。为探讨其分子机理,采用RT-PCR和RACE方法,克隆高羊茅叶片FaRVE8基因,结果显示,FaRVE8全长为1881 bp,有1236 bp的开放阅读框,编码411个氨基酸,同属于MYB样因子。遗传进化树表明其与禾本科植物二穗短柄草、节节麦、大麦的同源蛋白亲缘关系较近。利用荧光定量分析高羊茅叶片中FaRVE8在不同光照处理下的表达特征,结果表明,FaRVE8在不同光照处理下均有表达,且呈现出明显的昼夜节律。亚细胞定位显示FaRVE8定位在细胞核中,可能在细胞核中发挥重要作用。以上研究表明,FaRVE8在调节生物节律中有重要作用,为进一步探讨FaRVE8基因的功能和分子调控机制奠定基础。

The circadian clock is a complex regulatory network that promotes plant growth in the ever-changing environment.REVEILLE 8(RVE 8)is an important gene in the circadian clock.In order to explore the molecular mechanism,the FaRVE 8 gene of leaves from Festuca arundinacea was cloned by RT-PCR and RACE.It was found that the full length of FaRVE 8 was 1881 bp,the open reading frame was 1236 bp,encoding 411 amino acids,which belonged to a MYB-like factor.The phylogenetic analysis showed that it is closely related to Brachypodium distachyon,Aegilops tauschii,and Hordeum vulgare.Real-time fluorescence quantitative analysis of FaRVE 8 expression in F.arundinacea leaves under different light treatments showed that FaRVE 8 was expressed under different light treatments and showed obvious circadian rhythm.Subcellular localization indicated that FaRVE 8 is localized in the nucleus,and FaRVE 8 may play an important role in the nucleus.These above studies indicate that the FaRVE 8 gene plays an important role in regulating biological rhythms,laying the foundation for further study of the function and molecular regulation mechanisms of the FaRVE 8 gene.