视网膜母细胞瘤结合蛋白在非小细胞肺癌中的差异性表达

Differential expression of retinoblastoma-binding protein in non-small cell lung cancer

目的探讨视网膜母细胞瘤结合蛋白6(RBBP6)在非小细胞肺癌中的表达情况及其与临床病理特征的关系。方法采用Realtime PCR和逆转录RNA法检测非小细胞肺癌和癌旁新鲜组织样本中RBBP6 mRNA的表达差异,同时采用免疫组化法检测RBBP6在非小细胞肺癌和癌旁石蜡包埋组织中的表达情况,并分析RBBP6表达与患者临床及病理特征之间的相关性。结果RBBP6 mRNA在非小细胞肺癌中高表达,其中在高分化非小细胞肺癌中的表达显著高于低分化非小细胞肺癌(P<0.05)。RBBP6蛋白的表达强度与非小细胞肺癌分化程度呈正相关(Spearsman r=0.309,P<0.05),中分化和高分化非小细胞肺癌的RBBP6蛋白阳性率分别为96.9%和90%,而低分化非小细胞肺癌的RBBP6蛋白阳性率只有76%(χ^2=6.827,P<0.033),两者间比较差异显著。进一步分析发现,RBBP6蛋白表达与非小细胞肺腺癌的分化程度相关(P<0.05),与吸烟、年龄、性别等其他临床特征无关(P>0.05)。结论RBBP6 mRNA和蛋白均在高分化非小细胞肺癌中高表达,提示该基因很可能参与非小细胞肺癌的发生和发展。

Objective To investigate the expression of RBBP6 and its relation to clinicopathological features in non-small cell lung cancer.Methods The expression of RBBP6 mRNA in fresh frozen tissue samples of carcinoma and adjacent tissue were detected by reverse transcription-polymerase chain reaction(RT-PCR)and real-time quantitative polymerase chain reaction(RT-PCR).Expression of RBBP6 in the paraffin-embedded non-small cell lung cancer tissues was determined by immunohistochemistry,and its relationship with pathological features was analyzed.Results Detection of RBBP6 mRNA in non-small cell lung cancer showed that it was overexpressed in well differentiated cases,higher than that in poor differentiated ones(P=0.011).Combined with the clinical and pathologic features,the expression of RBBP6 protein was significantly associated with differentiation(Spearsman r=0.309,P=0.033).The positive expression rates of RBBP6 protein in well differentiated cases and medium differentiated ones were 96.9% and 90.0%,respectively.But it was 76.0% in poor differentiated cases(χ^2=6.827,P=0.033).Further analysis revealed that the expression of RBBP6 protein was highly correlated with the differentiation of non-small-cell lung adenocarcinoma(P=0.038).The expression of RBBP6 protein had no significant relationship with sex,age and smoking history.Conclusion RBBP6 protein and gene are highly expressed in non-small cell lung cancer that might participate in process of cell proliferation.Meanwhile,RBBP6 involves the regulation of cell differentiation in non-small cell lung cancer.These may affects the prognosis of patients;however,this finding needs to be further confirmed by the clinical follow-up data.