母源纳米硒对岢岚绒山羊后代睾丸发育的影响

Effect of maternalnano-selenium on testicular development of the offspring of Kelan cashmere goats

为了研究母源纳米硒对后代羔羊睾丸发育的影响,在哺乳母羊日粮中添加0.5 mg/kg的纳米硒,120 d哺乳期结束后,采用原子荧光和可见光分光光度法、巴氏染色法、TUNEL法和Real-time PCR法,检测母羊与羔羊供试样品的硒含量、抗氧化性能、精子形态、睾丸组织凋亡和凋亡基因表达。结果显示:纳米硒添加组(S组)的母羊及羔羊血液及母乳硒含量均极显著高于正常饲喂组(C组)(P<0.01);C组硒含量排序为母羊血硒>初乳>羔羊血硒>常乳,S组中硒含量高低比较为初乳>母羊血硒>羔羊血硒>常乳;C组和S组母羊组织硒的沉积顺序均为肾脏>肝脏>心肌;C组羔羊组织中硒的沉积顺序是肾脏>肝脏≈睾丸>心肌,S组羔羊组织硒的沉积顺序为睾丸>肾脏>肝脏>心肌;S组睾丸组织发育显著优于C组(P<0.05),S组精子畸形率极显著降低(P<0.01);S组羔羊睾丸组织中谷胱甘肽过氧化物酶(GSH-Px)的抗氧化活性极显著高于C组(P<0.01),超氧化物歧化酶(SOD)的活性显著提高(P<0.05),丙二醛(MDA)的浓度极显著降低(P<0.01);TUNEL检测S组羔羊睾丸组织细胞凋亡显著低于C组(P<0.05),促凋亡基因(Bax)的表达极显著降低(P<0.01)。结果表明:哺乳母羊日粮中添加纳米硒可以通过血乳屏障传递来提高后代公羔血液及睾丸组织的抗氧化力,促进睾丸组织发育,降低精子畸形率。

In order to identify the effect of maternal nano-selenium on the testicular development of offspring lambs,an experiment was conducted for 120 days by adding 0.5 mg/kg nano-selenium to the diet of lactating ewes.Then,the selenium content,oxidation resistance,sperm morphology,testicular tissue apoptosis and apoptosis gene expression of the ewes and their lambs were detected using atomic fluorescence and visible spectrophotometry,Papanicolaou staining,the TUNEL method and Real-time PCR.The results showed that the selenium contents in the blood and milk of the ewes and lambs in the nano-selenium supplemented group(the S Group)was significantly higher than those of the control group(the C Group)(P<0.01).The order of the selenium content in the C Group was ewe blood selenium>colostrum>lamb blood selenium>milk,and that of the S Group was colostrum>ewe blood selenium>lamb blood selenium>milk.The order of selenium deposition in the ewe tissues of the C Group and the S Group was kidney>liver>heart,that in the tissues of the C Group lambs was kidney>liver≈testis>heart,and that in the tissues of the S Group lambs was testisr>kidney>liver>heart.The testicular tissue development in the S Group was significantly better than that in the C Group(P<0.05),and the sperm malformation rate in the S Group was significantly lower(P<0.01).Compared with the C Group,the activity of cGPX in S Group was significantly higher(P<0.01),the activity of SOD was significantly increased(P<0.05),and the concentration of MDA was significantly decreased(P<0.01).The apoptosis of the testis tissue cells in the S Group detected with the TUNEL method was significantly lower than in the C Group(P<0.05),and the expression of proapoptotic gene Bax was significantly decreased(P<0.01).Taken together,nano-selenium addition to the diet of lactating ewes enhanced the antioxidant capacity of the blood and testicular tissues of the offspring lambs through the blood-milk barrier,promoted the development of testicular tissues,and reduced the rate of sperm malformation.