摘要
为了解新疆北疆地区硬蜱携带莱姆病病原伯氏疏螺旋体的流行现状,选择莱姆病病原伯氏疏螺旋体5S-23S rRNA基因间隔区设计嵌套式引物,建立了具有较高敏感性和特异性的伯氏疏螺旋体套式PCR检测方法,应用该方法对2018—2019年在新疆北疆6个地区采集的硬蜱进行伯氏疏螺旋体带菌率检测。结果显示:伯氏疏螺旋体总阳性率为15.17%(81/534),其中青河县阳性率最高,达23.64%(26/110)。提示:应进一步加强及重视北疆地区莱姆病的防控工作。
In order to understand the prevalence of Borrelia burgdorferi carried by ticks in the northern Xinjiang area,nested primers were designed in this study in the r5 S-23 SrRNA gene spacer of the Lyme disease pathogen,and a highly sensitive and specific method for nested PCR detection of Lyme disease was established.The ticks collected from six regions in the northern Xinjiang area in 2018-2019 were tested for Borrelia burgdorferi,with a total positive rate of 15.17%(81/534);and this positive rate was 23.64%(26/110)in Qinghe County in the area.Our study indicated that the prevention and control of Lyme disease in the northern Xinjiang area should be further strengthened and valued.
作者
王娅
刘丽娅
谢彩云
马晓菁
叶锋
谷文喜
马俊杰
钟旗
易新萍
WANG Ya;LIU Liya;XIE Caiyun;MA Xiaojing;YE Feng;GU Wenxi;MA Junjie;ZHONG Qi;YI Xinping(Veterinary Research Institute,Xinjiang Academy of Animal Sciences/Center of Clinical Veterinary Medicine Research,Xinjiang Academy of Animal Sciences,Urumqi 830000,China;College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830000,China)
出处
《畜牧与兽医》
北大核心
2020年第6期116-119,共4页
Animal Husbandry & Veterinary Medicine
基金
国家重点研发计划项目(2017YFC1200500)。
关键词
莱姆病
套式PCR
蜱
检测
Lyme disease
nested PCR
tick
detection
