血泊形态的时序性变化

Time Regularity of Morphology of Blood Pools

目的研究血泊整体干燥过程,监测血泊形态和质量变化,以期为血泊经过时间推断提供参考指标和理论依据。方法在清洁瓷板上制备4例15 mL人血泊样本,封闭、暗室、温度(20.0±0.5)℃、湿度35%~45%的环境下,动态观察0~60.0 h内血泊形态和质量变化,数码相机获取血泊图像,利用MATLAB R2014b计算血泊面积,Image J测量裂隙长度,SPSS 16.0统计分析。结果血泊整体干燥过程分为5个时期:凝固期(0~4.5 h)、凝胶期(>4.5~20.0 h)、胶-固混合期(>20.0~37.0 h)、固体期(>37.0~40.0 h)、完全干燥期(>40.0~45.0h)。0~45.0 h血泊质量随时间呈线性下降,>45.0~60.0 h质量随时间下降不明显。标准化质量(y2)与经过时间(x)呈现较好的相关性y2=0.018 2 x+0.271 4(R^2=0.967 9)。血泊面积变化率、边缘移动距离、放射状裂隙平均长度与经过时间相关性较低。结论血泊干燥整体形态变化随时间呈一定规律性,建立的标准化质量指标为血泊经过时间推断提供了参考依据。

Objective To provide reference indexes and theoretical basis for age estimation of blood pools by investigating the entire drying process and monitoring the change of morphology and mass.Methods Four 15 mL blood pool samples were prepared on the clean ceramic plate.The change of morphology and mass of blood pools in a closed dark environment with a temperature of(20.0±0.5)℃and a humidity of 35%-45%were dynamically observed from 0 h to 60 h.Images of the blood pools were recorded by digital camera.The area of blood pools was calculated by MATLAB R2014 b,the length of cracks was measured by Image J and the statistical analysis was performed by SPSS 16.0.Results By summarizing and analyzing,the drying of blood pools was divided into five stages:coagulation(0-4.5 h),gelation(>4.5-20.0 h),gel-solid mixing(>20.0-37.0 h),solid(>37.0-40.0 h)and final desiccation(>40.0-45.0 h).From 0 to 45 h,the mass of the blood pools decreased linearly with time,and the decrease was not obvious from 45.0 to 60.0 h.The standardized mass(y2)showed strong correlation with the time(x)y2=0.0182 x+0.2714(R^2=0.9679).The area change rate of blood pools,the distance that the edge of blood pools moved,the average length of radical cracks had little correlation with the time that passed.Conclusion The overall morphological characteristics of blood pools show a certain regularity with the time and the standardized indexes established provide a reference for the age estimation of blood pools.