生长分化因子5与发育性髋关节脱位的相关性研究

Correlation between growth differentiation factor 5 and congenital hip dislocation

目的探究生长分化因子5(GDF5)与发育性髋关节脱位(DDH)的相关性。方法选取新疆医科大学第一附属医院自2017年10月~2019年10月收治的DDH患儿48例作为观察组,另选择同期体检的健康儿42例作为对照组,两组小儿均使用TaqMan探针法进行单核苷酸基因多态性(SNP)位点分型,探讨SNP位点与DDH发病的相关性,另外按照DDH的严重程度进行分组,分别为先天性髋关节发育不良组(n=19)、先天性髋关节半脱位组(n=14)、完全性髋关节脱位组(n=15),探讨不同分类下DDH与SNP位点的相关性。结果观察组基因型频率TT、TC及CC与对照组比较,差异有统计学意义(P <0.05)。观察组等位基因型频率T、C与对照组比较,差异无统计学意义(P> 0.05)。观察组不同分类患儿的TT、TC及CC基因型频率比较,差异有统计学意义(P <0.05)。观察组不同分类患儿T、C等位基因型频率比较差异无统计学意义(P> 0.05)。完全性髋关节脱位组的T等位基因型频率是对照组的1.42倍,先天性髋关节半脱位组T等位基因型频率是对照组的1.36倍,完全性髋关节脱位组的T等位基因型频率是先天性髋关节半脱位组1.65倍(P <0.05)。结论 GDF5在DDH的发生发展中具有重要的作用,存在明显的相关性。

Objective To explore and analyze the correlation between growth differentiation factor 5(GDF5)and pediatric developmental dysplasia of the hip(DDH).Methods Forty-eight children with congenital hip dislocation treated in the First Affiliated Hospital of Xinjiang Medical University from October 2017 to October 2019 were selected as the observation group,and 42 healthy children checked the same period were selected as the control group.Single nucleotide gene polymorphism(SNP)site typing was investigated by TaqMan probe in both groups,the correlation between SNP and DDH.In addition,according to the severity of congenital dislocation of hip joint,they were grouped as congenital dysplasia group(n=19),congenital hip subluxation group(n=14),and complete hip dislocation group(n=15),the correlation between congenital dislocation of hip joint and SNP site under different classifications was explored.Results There were statistically significant differences in genotype frequencies TT,TC and CC between the observation group and the control group(P<0.05).There was no significant difference in allele frequency T and C between the observation group and the control group(P>0.05).There were statistically significant differences in genotype frequencies of children in different categories in the observation group between TT,TC and CC(P<0.05).There were no significant differences in allele frequency T and C among children in different categories in the observation group(P>0.05).The frequency of T alleles in the complete hip dislocation group was 1.42 times that of the control group,the frequency of T alleles in the congenital hip subluxation group was 1.36 times of the control group,the complete hip dislocation group of the T allele frequency was 1.65 times of the congenital hip subluxation group(P<0.05).Conclusion GDF5 plays an important role in the occurrence and development of DDH,and it has obvious correlation.