微量元素对川芎活性成分的影响

Effects of Trace Elements on the Active Constituents of Ligusticum chuanxiong Hort.

采用“3414”试验设计进行盆栽试验,研究叶面喷施B(硼)、Zn(锌)、Fe(铁)3种微量元素对川芎绿原酸、阿魏酸、阿魏酸松柏酯、藁本内酯及洋川芎内酯A 5种活性成分积累的影响,为川芎栽培中微量元素平衡施肥技术的形成奠定基础。结果表明,B不利于阿魏酸松柏酯、藁本内酯及洋川芎内酯A的积累,低质量浓度B(1 g/L)可促进绿原酸及阿魏酸积累,分别比不施B增加6.56%、2.22%,用量过高反而存在抑制作用。Zn不利于绿原酸、阿魏酸松柏酯、藁本内酯及洋川芎内酯A积累,高质量浓度Zn(4 g/L)水平下阿魏酸含量比不施Zn增加33.70%。低质量浓度Fe(1 g/L)水平下阿魏酸松柏酯、藁本内酯、洋川芎内酯A含量分别比不施Fe高5.34%、9.79%、12.23%;高质量浓度Fe(4 g/L)水平下绿原酸、阿魏酸含量分别比不施Fe处理高8.46%、6.98%。B、Zn对川芎5种活性成分积累存在负交互作用;B、Fe对阿魏酸积累存在负交互作用,对其他4种活性成分存在正交互作用;Zn、Fe对绿原酸、藁本内酯及洋川芎内酯A积累存在正交互作用,对阿魏酸及阿魏酸松柏酯积累存在负交互作用。故叶面喷施B、Zn、Fe肥未必能有效增加川芎活性成分含量,需控制好用量以免产生不利影响,栽培中应尽量少施或不施。

The“3414”experimental design was used to carry out a pot experiment,in order to study the effects of three trace elements of B(boron),Zn(zinc),and Fe(iron)foliar application on chlorogenic acid,ferulic acid,coniferyl ferulate,ligustilide and senkyunolide A accumulating of Ligusticum chuanxiong Hort.(Hereinafter referred to as Chuanxiong),which could lay a foundation for the formation of trace elements balanced fertilization techniques for chuanxiong.Results as follows:B was not conducive to the accumulation of coniferyl ferulate,ligustilide and senkyunolide A.Low concentration of B(1 g/L)could promote the accumulation of chlorogenic acid and ferulic acid,and the chlorogenic acid and ferulic acid content was 6.56%and 2.22%respectively higher than that without B.Excessive use of B had inhibitory effects.Zn was not conducive to the accumulation of chlorogenic acid,coniferyl ferulate,ligustilide and senkyunolide A.High concentration of Zn(4 g/L)could increase the ferulic acid content with 33.70%higher than that without Zn.The contents of coniferyl ferulate,ligustilide and senkyunolide A at low concentration of Fe(1 g/L)were respectively 5.34%,9.79%and 12.23%higher than those without Fe.The contents of chlorogenic acid and ferulic acid at high concentration of Fe(4 g/L)were respectively 8.46%and 6.98%higher than those without Fe.B and Zn had a negative interaction on the accumulation of 5 active ingredients of chuanxiong.B and Fe had a negative interaction on the accumulation of ferulic acid and a positive interaction on the other 4 active ingredients.Zn and Fe had a positive interaction on the accumulation of chlorogenic acid,ligustilide and senkyunolide A,and a negative interaction on the accumulation of ferulic acid and coniferyl ferulate.Therefore,foliar application of B,Zn and Fe fertilizers might not be effective in increasing the active ingredients content of chuanxiong.It was necessary to control the dosage so as not to have adverse effects.It should be not applied or as little as possible during cultivation.