免疫球蛋白样转录物5在胶质母细胞瘤中的表达及对患者预后的评估价值

Expression of immunoglobulin-like transcript 5 in glioblastoma and its value in evaluating prognosis

目的探讨免疫球蛋白样转录物5(ILT5)在胶质母细胞瘤中的表达情况,分析其与患者临床特征的关系及对预后的评估价值。方法取92例胶质母细胞瘤患者的胶质瘤组织,同期取40例行正常脑组织切除减压术患者的正常脑组织。采用实时荧光聚合酶链反应(PCR)检测ILT5 mRNA的相对表达量;免疫组化法检测ILT5蛋白的表达情况,并分析其与患者临床特征及预后的关系。结果胶质瘤组织中ILT5 mRNA的相对表达量和ILT5蛋白表达率均低于正常脑组织,差异有统计学意义(P<0.05)。不同年龄、组织学分级、肿瘤直径和肿瘤部位ILT5 mRNA的相对表达量比较,差异均有统计学意义(P<0.05)。不同年龄、组织学分级及肿瘤直径胶质母细胞瘤患者ILT5蛋白表达情况比较,差异均有统计学意义(P<0.05)。ILT5蛋白低表达患者中位生存时间为20.1个月(95%CI:17.4~22.8个月),短于ILT5蛋白高表达患者的28.1个月(95%CI:25.3~31.0个月);ILT5蛋白高表达患者3年生存率44.1%,高于ILT5蛋白低表达患者的22.4%,差异均有统计学意义(P<0.05)。结论ILT5在胶质母细胞瘤中低表达,可能与年龄、WHO分级、肿瘤直径及预后等密切相关,有望成为胶质母细胞瘤诊疗的新靶点。

Objective To investigate the expression of immunoglobulin-like transcript 5(ILT5)in glioblastoma,and to analyze its relationship with clinical features and its value in prognosis evaluation.Method The tumor tissues of 92 patients with glioblastoma were collected,and the normal brain tissues of 40 patients treated with brain decompression surgery were collected in the meantime.The relative expression levels of ILT5 mRNA were detected by real-time fluorescent PCR,and ITL5 protein expression by immunohistochemistry,to investigate their relationship with the clinical features and prognosis.Result Compared with the normal brain tissues,the relative expression of ILT5 mRNA and the expression rate of ILT5 protein in glioma tissues were significantly decreased,the difference was statistically significant(P<0.05).The relative expression of ILT5 mRNA and ILT5 protein were significantly different in glioblastoma patients of different ages,with varying histological classification and tumor diameter(P<0.05).The median survival time of patients with low expression of ILT5 protein was 20.1 months(95%CI:17.4-22.8),which was decreased compared to the 28.1 months(95%CI:25.3-31.0)in patients with high ILT5 protein expression(P<0.05).The 3-year survival rate in patients with high ILT5 expression was 44.1%versus 22.4%in those with low ILT5 protein expression(P<0.05).Conclusion The expression of ILT5 gene is low in patients with glioblastoma,and is possibly related to age,WHO classification,tumor diameter and prognosis,therefore it is expected to become a new target for the diagnosis and treatment of glioblastoma.