H3K27M和Ki-67在小儿脑胶质瘤中的表达及相关性

Expression and correlation of H3K27M and Ki-67 in pediatric glioma

目的:探讨H3K27M和Ki-67在小儿脑胶质瘤组织中的表达及意义。方法:收集2013年11月至2018年6月清华大学玉泉医院38例小儿脑胶质瘤和10例正常脑组织,采用免疫组织化学检测H3K27M和Ki-67表达并结合相关统计学方法进行分析。结果:H3K27M阳性表达与性别、年龄、肿瘤部位无显著性差异(P>0.05),但与肿瘤病理级别有显著性相关(P<0.05)。Ki-67在胶质瘤中阳性表达率为100%,Ki-67高标记指数与性别、年龄无显著性相关(P>0.05),与病理级别、肿瘤部位有显著性相关(P<0.05)。患儿的性别、年龄与生存时间之间无显著性差异(P>0.05),病理分级、肿瘤部位、H3K27M蛋白阳性、Ki-67高标记指数在患儿生存时间之间有显著性差异(P<0.05)。结论:小儿脑胶质瘤中H3K27M、Ki-67的表达水平对小儿脑胶质瘤的病理分级、预后具有提示意义,且H3K27M、Ki-67蛋白在胶质瘤组织中的表达具有相关性,为研究胶质瘤的发生发展及靶向治疗提供了线索。

Objective:To investigate the expression and significance of H3K27M and Ki-67 in pediatric glioma.Methods:Immunohistochemistry was used to detect the expression of H3K27M and Ki-67 in 38 patients with pediatric glioma and 10 patients with normal brain tissue,who enrolled in Yuquan Hospital,Tsinghua University from November 2013 to June 2018.The results were analyzed using appropriate statistical methods.Results:The positive expression of H3K27M was not significantly correlated with gender,age,and tumor site(P>0.05).However,it was significantly correlated with the tumor pathological grade(P<0.05).The positive expression rate of Ki-67 in pediatric glioma was 100%.The high label index of Ki-67 was not significantly correlated with gender and age(P>0.05).However,it was significantly correlated with the pathological grade and tumor site(P<0.05).There were no significant correlations between the ptients'gender,age,and survival time(P>0.05).There were significant correlations between the tumor pathological grade,tumor location,H3K27M protein-positive,Ki-67 high label index,and patient survival time(P<0.05).Conclusions:The expression level of H3K27M and Ki-67 appeared to be related to the pathological grade and prognosis of pediatric glioma.Besides,the expression of H3K27M and Ki-67 protein in glioma tissue were correlated.This could be useful for studying the occurrence and development of glioma as well as investigating potential targeted therapy options.