白皮杉醇对牙周膜干细胞成骨分化能力的影响

Effect of Piceatannol on the osteogenic differentiation of human periodontal ligament stem cells

目的探讨不同浓度白皮杉醇对牙周膜干细胞(hPDLSCs)成骨分化能力的影响。方法通过有限稀释法获得人的牙周膜干细胞,分别用浓度为0.16、0.8、4、20、50、100μmol/L的白皮杉醇处理牙周膜干细胞72 h后,检测白皮杉醇对牙周膜干细胞的细胞毒性。取第4代的牙周膜干细胞,分别用0.16、0.8、4、20μmol/L的白皮杉醇+DMEM处理牙周膜干细胞,以DMEM组为阴性对照,成骨诱导液组为阳性对照。连续培养牙周膜干细胞21 d后,对各组细胞进行茜素红染色,检测牙周膜干细胞成骨分化的结果;用乙酸将矿化结节溶解后,用酶标仪进行定量检测。结果与空白对照组相比较,0.16、0.8、4、20μmol/L的白皮杉醇对牙周膜干细胞没有细胞毒性(P>0.05);50、100μmol/L的白皮杉醇对牙周膜干细胞有明显的细胞毒性(P<0.05)。0.16、0.8、4、20μmol/L的白皮杉醇处理组以及成骨诱导组均能观察到矿化结节的产生,不同浓度的白皮杉醇处理组组间矿化结节形成量没有统计学差异(P>0.05)。结论在一定剂量范围内白皮杉醇能够促进牙周膜干细胞的成骨向分化,但没有剂量依赖性。

Objective To investigate the effect of different concentration of piceatannol on the osteogenic differentiation of human periodontal ligament stem cells.Methods Human periodontal ligament stem cells were isolated with limited dilution methods.Cells were treated in different concentration of piceatannol for 72h,and then the cell viabilities were evaluated by MTT assays.The human periodontal ligament stem cells were cultured with DMEM and 0.16,0.8,4,20μmol/L piceatannol for 21 days and stained with Alizarin Red S.Then the Alizarin Red S dye was dissolved and measured at 405 nm.Results Compared with the control,0.16,0.8,4 and 20μmol/L piceatannol had no cytotoxic on hPDLSCs.But the concentration of 50 and 100μmol/L piceatannol had cytotoxicity.After treated with the concentration of 0.16,0.8,4 and 20μmol/L piceatannol for 21 days,the mineralized nodules formation in hPDLSCs was detected.However,there was no significant difference in the number of mineralized nodules between different groups(P>0.05).Conclusion Piceatannol promotes the osteogenic differentiation of hPDLSCs,but it is not in a dose-dependent way.