辣木源多肽组分改善D-半乳糖致衰小鼠氧化损伤作用

Peptide Fractions from Moringa oleifera Ameliorate D-Galactose-Induced Oxidative Damage in Mice

为评价辣木源多肽组分在动物体内的抗氧化功效,本实验采用酶法、乙醇分级沉淀法制备辣木叶多肽组分(MOL-P)、辣木籽多肽组分(MOS-P),构建D-半乳糖致衰小鼠模型,考察MOL-P和MOS-P在低、高剂量(200、500 mg/(kg mb·d))下连续灌胃小鼠8周,对衰老小鼠体质量,肝脏和脑的脏器系数,血清、肝脏和脑抗氧化酶活力和丙二醛(malondialdehyde,MDA)水平的影响。结果表明:MOL-P和MOS-P均能上调衰老小鼠脑系数至正常组水平(P>0.05);辣木源多肽组分能显著提高衰老小鼠血清谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和肝脏超氧化物歧化酶(superoxide dismutase,SOD)活力(P<0.05),一定程度上降低血清、肝脏和脑组织MDA水平,其中低剂量MOL-P和高剂量MOS-P对血清GSH-Px活力的提升作用最好(分别比模型组提高了35.80%和36.97%);MOS-P对肝脏SOD活力提升作用最好,高、低剂量组分别比模型组提高了26.08%和34.20%;高剂量MOL-P和高剂量MOS-P对脑组织脂质过氧化的抑制作用最好(高剂量MOL-P和高剂量MOS-P脑组织MDA水平分别比模型组降低了41.28%和44.95%);MOL-P能将血清、肝脏MDA水平下调至正常组水平(P>0.05)。采用苏木精-伊红染色观察衰老小鼠肝脏组织损伤程度,结果表明,MOL-P和MOS-P均能有效缓解D-半乳糖所致肝脏氧化损伤。结论:辣木叶、辣木籽多肽组分均能在动物体内拮抗氧化应激,具有良好的抗衰老活性,可作为功能性因子用于抗衰老食品的研制。

In order to evaluate the antioxidant activity of peptide fractions from Moringa oleifera,peptides from Moringa oleifera leaves(MOL-P)and seeds(MOS-P)were prepared by enzymatic hydrolysis followed by fractional ethanol precipitation.A D-galactose-induced aging mouse model was established to assess the effects of administration with MOL-P and MOS-P at low and high doses(200 and 500 mg)/(kg mb·d)by gavaging for 8 weeks on body mass,liver and brain indices,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities and malondialdehyde(MDA)levels in serum,liver and brain of aging mice.The results indicated that MOL-P and MOS-P could restore brain indices close to normal(P>0.05).Compared with the model group,both of them could significantly improve GSH-Px activity in serum and SOD activity in liver(P<0.05),and reduce MDA levels in serum,liver and brain.Low-dose MOL-P and high-dose MOS-P had the best effects on improving GSH-Px activity in serum(higher than that of the model group by 35.80%and 36.97%,respectively).MOS-P had the best effect on the improvement of liver SOD activity,at high and low doses increasing liver SOD activity by 26.08%and 34.20%compared with the model group,respectively.MOL-P and MOS-P both at high dose showed the strongest inhibitory effects on lipid peroxidation in brain tissue(MDA levels were lower than that of the model group by 41.28%and 44.95%,respectively).In addition,MOL-P was able to reduce MDA levels in serum and liver to the normal level(P>0.05).Both MOL-P and MOS-P could effectively alleviate oxidative damage in liver caused by D-galactose as evaluated by hematoxylin-eosin(HE)staining.Therefore,the peptides from Moringa oleifera leaves and seeds can antagonize oxidative stress in vivo and have good anti-aging effects,making them potential functional ingredients in anti-aging foods.