色谱及色谱-质谱技术在复杂基质中赤霉素痕量分析中的应用

Chromatography Alone and Coupled with Mass Spectrometry for the Determination of Trace Gibberellins in Complex Matrices:A Review

赤霉素(gibberellins,GAs)是一类在植物体内广泛存在的植物激素,在植物生长、发育以及对环境胁迫的反应中起着至关重要的作用。GAs是一类四环二萜酸,其基本结构是二十碳赤霉烷。到目前为止,已发现的GAs多达136种。随着近年来GAs在农业生产中的广泛使用以及相关毒性的深入研究,由GAs引起的食品安全问题得到越来越多的关注。GAs精准分析技术有助于深入解析GAs代谢行为及其生理活性与毒性。由于GAs的不稳定性及其极低的含量(一般为ng/g,甚至为pg/g水平),复杂基质中GAs分析的前处理技术和分析手段经历了不断更新与提升的过程。本文综述了过去10年GAs痕量分析中前处理技术和分析手段上的发展与突破,并展望了复杂基质中GAs分析技术发展趋势。

Gibberellins(GAs) are a class of plant hormones that are widely found in plants, which play a crucial role in plant growth, development, and response to environmental stress. GAs are a class of tetracyclic diterpenic acids whose basic structure is 20-carbon gibberellin. So far, 136 GAs have been found. As GAs have been extensively applied in agricultural products and intensive research efforts have been made on their toxicity in recent years, their potential to cause food safety issues has become a subject of growing concern. Accurate analysis of GAs are helpful for better understanding their metabolism, physiological activity and toxicity. Due to their instability and extremely low content(generally ng/g, even pg/g levels), the pretreatment techniques and analytical methods for the detection of GAs in complex matrices are being continuously updating. This article reviews the development and breakthroughs of pretreatment techniques and analytical methods for trace analysis of GAs over the past 10 years, and future development trends are discussed.