消渴平含药血清对高糖诱导的大鼠肾小球系膜细胞增殖、周期及TGF-β1的影响

Effects of Serum Containing Xiaokeping(消渴平)on Proliferation,Cell Cycle and TGF-β1 Expression of Rat’s Mesangial Cells Induced by High Glucose

目的:观察消渴平(xiaokeping,XKP)含药血清对高糖诱导的大鼠肾小球系膜细胞增殖、周期及转化生长因子(TGF-β1)表达的影响,探讨其治疗DKD的作用机理。方法:使用不同浓度的消渴平含药血清及厄贝沙坦含药血清与高糖共同培养大鼠系膜细胞,应用MTT法观察各组细胞增殖情况,流式细胞仪检测细胞周期变化,Western-blot及RT-PCR检测TGF-β1蛋白和mRNA表达情况。结果:与空白对照组相比,高糖组系膜细胞增殖明显增加(P<0.01),消渴平可呈浓度依赖性地抑制系膜细胞增殖。细胞周期检测结果显示,与高糖组相比,消渴平高剂量组可明显降低S期系膜细胞的百分比(P<0.05),Western-blot及RT-PCR检测显示消渴平可显著减少TGF-β1表达(P<0.05)。结论:消渴平可明显抑制高糖诱导的大鼠系膜细胞增殖,阻滞细胞周期,降低TGF-β1表达,这可能是其防治DKD的机制之一。

Objective:To observe the effects of serum containing Xiaokeping(XKP)on the proliferation,cell cycle and TGF-β1 expression of rat’s mesangial cells induced by high glucose,and to explore the mechanism of its prevention and treatment of diabetic kidney disease.Methods:Rat mesangial cells were co-cultured with different concentrations of XKP and Irbesartan drug serum and high glucose.Cell proliferation in each group was observed by MTT assay,cell cycle changes were detected by flow cytometry,and the expression of TGF-β1 protein and mRNA were detected by Western blot and RT-PCR.Results:Compared with the blank control group,the proliferation of mesangial cells was significantly increased in the high-glucose group(P<0.01),and XKP could inhibit the proliferation in a concentration-dependent way.Compared with the high glucose group,the percentage of s-phase mesangial cells was significantly reduced in the high dose group of XKP(P<0.05),and the expression of TGF-β1 was significantly reduced(P<0.05).Conclusion:XKP can significantly inhibit the proliferation of mesangial cells induced by high glucose,block the cell cycle,and down-regulate the expression of TGF-β1,which may be one of the mechanisms of its prevention and treatment of diabetic kidney disease.