虾夷扇贝闭壳肌蛋白溶解性与三磷酸腺苷的关联性

A Relevance of Adductor Protein Solubility to ATP in Yesso Scallop Patinopecten yessoensis

刚采捕的活品虾夷扇贝立即进行4℃冷却干藏,分别于0、48、72、96、120 h采5只样本,分离出横纹肌与平滑肌,测定其三磷酸腺苷及其关联化合物含量,同时测定贮藏0、48、120 h扇贝肌肉的蛋白溶解性,以探究肌肉蛋白溶解性与扇贝活品品质的关系。试验结果表明,冷却干藏期间扇贝活力呈下降趋势,三磷酸腺苷在贮藏120 h后几乎消耗殆尽,而蛋白溶解性在高离子浓度范围内有明显上升趋势,120 h后表现出最好的溶解性。此外,三磷酸腺苷酶活性受到乙二醇双(2-氨基乙基醚)四乙酸(EGTA)抑制时肌肉匀浆表现较好的溶解性,碎肉经漂洗处理会导致肌肉三磷酸腺苷含量下降,肌原纤维蛋白溶解性升高。最后,对比肌肉均质前后三磷酸腺苷含量,发现均质处理导致肌肉三磷酸腺苷全部降解。虾夷扇贝闭壳肌蛋白质的溶出机制受三磷酸腺苷影响,对于捕后早期活力状态较好的扇贝,其三磷酸腺苷含量与蛋白溶解性的关联需要进一步系统探索。

Freshly harvested live Yesso scallop Patinopecten yessoensis were immediately cooled and stored at 4℃with air exposure.The striated muscle and smooth muscle were separated,and the contents of adenosine triphosphate and related compounds were determined in 5 samples collected at 0 h,48 h,72 h,96 h,and 120 h storage,and the protein solubility of the scallop muscle stored at 0 h,48 h,and 120 h were measured to investigate the the relevance of protein solubility and quality of live scallop.The results showed that Yesso scallop had decrease in vitality during air exposure storage,almost exhausted ATP after 120 h storage,and increase in the solubility of protein,especially in the higher ion concentration,with the maximal solubility in 120 h storage.However,higher solubility was observed when ATPase was inhibited with addition of ethylene glycol bis(2-aminoethyl ether)tetraacetic acid(EGTA).ATP was partially removed by rinsing minced muscle,along with the increasing myofibrillar protein solubility.Homogenization led to the degradation of ATP in muscle,and ATP was involved in the mechanism of muscle protein solubility.Further research was required to clarify the relationship between ATP content and muscle protein solubility property in early post-harvest active scallop.