产甲烷菌抑制剂对便秘小鼠结肠微生物菌群及代谢产物的影响

Effects of methanogen inhibitor on the composition of gut microbiota and short-chain fatty acids in mice with constipation

为研究产甲烷菌抑制剂(溴氯甲烷)对便秘小鼠的肠道微生物菌群及代谢产物的影响,选取60只5周龄雄性KM小鼠,随机分为3组:对照组、便秘组、甲烷抑制剂干预组.试验结束,采集小鼠结肠内容物通过高通量测序技术分析肠道菌群;气相色谱分析结肠内容物中短链脂肪酸含量;Real-time PCR技术分析肠道总菌及产甲烷菌数量变化.结果表明,溴氯甲烷处理后小鼠粪便颗粒数及粪便含水率显著增加,结肠内容物Lactobacillus及Streptococcus的相对丰度显著增加,Lachnospiraceae NK4a136 group,Lachnospiraceae_uncultured,Bacteroides,Muribaculaceae_norank及Staphylococcus的相对丰度显著降低,结肠菌群结构发生显著变化,产甲烷菌数量显著降低,但对总菌数量无显著影响.溴氯甲烷处理组总短链脂肪酸含量显著降低.本研究表明便秘小鼠在甲烷抑制剂干预后,结肠菌群结构及代谢产物均受到显著影响.

In this study,we aimed to evaluate the effects of a methanogen inhibitor,Bromochloromethane(BCM),on the composition of gut microbiota and short-chain fatty acids in mice with constipation induced by loperamide.Sixty Kunming mice were randomly divided into three groups as follows:normal control group,constipation group,and BCM group.At the end of the experiment,the colonic microbiota composition was analyzed using pyro-sequencing of 16S rRNA genes and real-time PCR,and the metabolites were further profiled through gas chromatography.The results revealed that the BCM group had significantly altered bacterial community composition.BCM treatment significantly increased the defection number and the water content of the feces.BCM treatment increased the abundance of Lactobacillus and Streptococcus,while decreased the abundance of Lachnospiraceae NK4a136,Lachnospiraceae_uncultured,Bacteroides,Muribaculaceae_norank,and Staphylococcus in the colon.BCM treatment also decreased the abundance of methanogens,but did not affect the total colonic bacterial counts.BCM treatment decreased the concentration of short chain fatty acids.In conclusion,BCM treatment significantly altered the microbiota and metabolite profile in the colon of mice with constipation.