流式细胞仪在造血干细胞生物学研究中的应用

Application of flow cytometerinstudy of hematopoieticstemcell biology

介绍了流式细胞仪的构造及其工作原理,阐述了流式细胞仪研究造血干细胞的原理,归纳了流式细胞仪和流式细胞术在造血干细胞造血功能的研究、靶基因对造血干细胞命运的研究、造血干细胞细胞周期的研究和药物等因素对造血干细胞分化功能的影响中的应用。比较了6种不同型号的流式细胞仪在造血干细胞分析和分选应用中的差异,分析了影响造血干细胞流式分析、分选的因素,以期为多激光多色流式分析和高速、高通量流式分选技术的研究提供思路。

The construction of the flow cytometer and its working principles were introduced.The application of flow cytometer to studying hematopoietic stem cells had its principles described,which involved in the studies on the hematopoietic function of hematopoietic stem cells,the effect of target gene on the fate of hematopoietic stem cells,cell cycle of hematopoietic stem cells and the influences of medicine and other factors on differentiation of hematopoietic stem cells.Six models of flow cytometer were compared for their application in the analysis and sorting of hematopoietic stem cells.The factors influencing the analysis and sorting of hematopoietic stem cells were explored to facilitate the study of multi-laser multicolor flow analysis and high-speed,high-throughput flow sorting techniques.