基于UPLC-Q-TOF/MS法野马追化学成分分析鉴定

Analysis of chemical constituents in Eupatorium lindleyanum by UPLC-Q-TOF/MS

目的应用超高效液相色谱-四级杆串联飞行时间质谱法(UPLC-Q-TOF/MS)对野马追药材的主要化学成分进行定性研究。方法采用Waters Acquity UPLC BEH C18(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸水(A)-乙腈(B)溶液为流动相,梯度洗脱,体积流量0.2 mL/min,柱温为35℃;质谱分析采用信息关联模式(IDA)正、负离子分别采集。结果应用PeakView软件的Formula Finder等功能、在线数据库及各色谱峰的二级碎片裂解规律,从野马追醇提样品中共鉴定出26个化合物,其中11个成分为野马追中首次报道;其主要成分类别包括黄酮、核苷、生物碱、苯丙素、倍半萜、香豆素、多元醇等。结论该方法精确、可靠、高效,适用于野马追化学成分的快速鉴定,为阐明野马追的药效物质基础提供科学依据。

Objective To analyze and identify the chemical constituents from Lindley eupatorium by using UPLC-Q-TOF/MS.Methods The separation was performed on Waters Acquity UPLC BEH C18(100 mm×2.1 mm,1.7μm)column with gradient elution of 0.1%formic acid(A)-acetonitrile(B),the flow rate was 0.2 mL/min.The column temperature was set at 35℃.The MS analysis was based on information associated mode(IDA),and positive and negative ions were collected respectively.Results A total of 26 compounds in L.eupatorium were identified by PeakView,combined with the mass spectrometry data of each chromatographic peak in the database,and the cleavage law of secondary fragment of each peak of which11 compounds were first reported for L.eupatorium.The main chemical constituents included flavonoids,nucleosides,alkaloids,phenylpropanoid,sesquiterpenoids,coumarins,polyols,etc.Conclusion The method is accurate,reliable and effficient,which is suitable for rapid identification of ingredients in L.eupatorium,which provides a reference for clarify its efficacy and material basis.