陕产珠子参地上部分挥发油的GC-MS分析

GC-MS Analysis of Essential Oil Extracted from Aboveground Part of Panax japonicus from Shaanxi Province

目的:研究陕产珠子参地上部分的挥发油组成;方法:利用水蒸气蒸馏法提取陕产珠子参地上部分的挥发油,采用气相色谱-质谱联用仪(GC-MS)分析挥发油中的化学成分;结果:三个产地的珠子参地上部分挥发油中共鉴别化学成分20个。凤县产珠子参地上部分挥发油含量约为0.20%,鉴定11种成分,主要成分为植酮(30.92%)、1,1,7-trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azulen-7-ol(19.59%)、α-芹子烯(15.82%)、5a-(hydroxymethyl)-5,7-dimethyl-2,3,4,5,5a,6,7,9a-octahydrospiro[2,5-methanobenzo[b]oxepine-10,2-oxiran]-3-ol(6.33%)、2-[(Z)-十八碳-9-烯氧基]乙醇(6.12%);眉县产珠子参地上部分挥发油含量约为0.22%,鉴定9种成分,主要成分为植酮(26.03%)、叶绿醇(19.50%)、α-芹子烯(13.57%)、1,1,7-trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azulen-7-ol(11.51%)、natural verticillol(9.88%);太白县产珠子参地上部分挥发油含量约为0.20%,鉴定10种成分,主要成分为植酮(37.68%)、α-himachalene(26.56%)、T-杜松醇(8.29%)、1-hydroxy-1,2-dihydrolycopene(6.22%),1,1,7-trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azulen-7-ol(4.90%)。其中:5-8,11-dihydroxy-10a,12a-dimethylhexadecahydro-1H-naphtho[1,2-h]chromen-1-yran-2-one(2.24%)、5a-(hydroxymethyl)-5,7-dimethyl-2,3,4,5,5a,6,7,9a-octahydrospiro[2,5-methanobenzo[b]oxepine-10,2-oxiran]-3-ol(6.33%)、β-愈创木烯(4.69%)、7,7,8,8-tetramethyloctahydro-2,3b-methanocyclopena[1,3]cyclopropa[1,2]benzene-4-ol(5.51%)、虾青素(2.35%)、2-[(Z)-十八碳-9-烯氧基]乙醇(6.12%)为凤县产珠子参的地上部分挥发油中的特有成分,natural verticillol(9.88%)、(2E,4E,6E,8E,10E)-8a-acetoxy-3a,6b-dihydroxy-3-(hydroxymethyl)-1,1,5,7-tetramethyl-4-oxo-1,1a,1b,3a,4,6a,6b,7,8,8a-decahydrocyclopenta[a]cyclopropa[f]naphthalen-8-yl tetradeca-2,4,6,8,10-pentaenoate(3.09%)、叶绿醇(19.50%)为眉县产珠子参地上部分挥发油中的特有成分,石竹素(3.58%)、视黄醛(3.54%)、α-himachalene(26.56%)、T-杜松醇(8.29%)、3α-Aethyl-5α-androstanol-(3β)-on-(17)(1.32%)为太白县产珠子参地上部分挥发油中的特有成分。结论:陕产珠子参地上部分挥发油含量均较低,三个产地的挥发油种类存在差异,且各地挥发油中均含有其特有成分,本研究为珠子参地上部分进一步开发研究提供参考。

Objective Study on the components of essential oil extracted from the aboveground part of Panax japonicus from Shaanxi Province.Methods the essential oil was extracted by steam distillation,and the chemical constituents were identified by gas chromatography-mass spectrometry(GC-MS).Results 20 components were identified from the aboveground parts of Panax japonicus from 3 producing areas.The content of volatile oil in aboveground parts of Panax japonicus from Feng County is about 0.20%.Eleven components have been identified,the main of which are Phytone(30.92%),1,1,7-trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azulen-7-ol(15.59%),α-Selinene(15.82%),5a-(hydroxymethyl)-5,7-dimethyl-2,3,4,5,5a,6,7,9a-octahydrospiro[2,5-methanobenzo[b]oxepine-10,2-oxiran]-3-ol(6.33%),(Z)-2-(9-octadecenyloxy)ethanol(6.12%);The content of essential oil in the aboveground part of Panax japonicus in Mei County was about 0.22%,Nine components were identified.The main components were Phytone(26.03%),3,7,11,15-tetramethyl-2-hexadecen-1-ol(19.50%),α-Selinene(13.57%),1,1,7-trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azule-7-ol(11.51%),natural verticillol(9.88%);The content of essential oil in the aboveground part of Panax japonicu in Taibai County was about 0.20%.Ten components were identified.The main components were Phytone(37.68%),α-himachalene(26.56%),(1R,4S,4aR,8aR)-4-isopropyl-1,6-dimethyl-1,2,3,4,4a,7,8,8a-octahydronaphthalen-1-ol(8.29%),1-hydroxy-1,2-dihydrolycopene(6.22%),1,1,7-trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azulen-7-ol(4.90%).5-8,11-dihydroxy-10a,12a-dimethylhexadecahydro-1H-naphtho[1,2-h]chromen-1-yran-2-one(2.24%),5a-(hydroxymethyl)-5,7-dimethyl-2,3,4,5,5a,6,7,9a-octahydrospiro[2,5-methanobenzo[b]oxepine-10,2-oxiran]-3-ol(6.33%),β-guaiacene(4.69%),7,7,8,8-tetramethyloctahydro-2,3b-methanocyclopena[1,3]cyclopropa[1,2]benzene-4-ol(5.51%),astaxanthin(2.35%),2-[(Z)-octadecyl-9-enoxy]ethanol(6.12%)are the unique components in the volatile oil of the aboveground part of Panax japonica produced in Feng county,natural verticillol(9.88%),(2E,4E,6E,8E,10E)-8a-acetoxy-3a,6b-dihydroxy-3-(hydroxymethyl)-1,1,5,7-tetramethyl-4-oxo-1,1a,1b,3a,4,6a,6b,7,8,8a-decahydrocyclopenta[a]cyclopropa[f]naphthalen-8-yl tetradeca-2,4,6,8,10-pentaenoate(3.09%),chlorophyllin(19.50%)are the typical components in the volatile oil of Panax japonica produced in Mei County,caryophyllin(3.58%),retinaldehyde(3.54%),α-himachalene(26.56%),T-juniol(8.29%),3α-Aethyl-5α-androstanol-(3β)-on-(17)(1.32%)are the characteristic components in the volatile oil of Panax japonica produced in Taibai County.Conclusion the content of essential oil in the aboveground part of Panax japonicu produced in Shaanxi is low,the types of volatile oil in the three producing areas are different,and the essentia oil contains its unique components in different places.This study provides a reference for the further development and research of the above ground part of Panax japonica.