基于激光诱导击穿光谱技术分析鳕鱼中8种元素含量

Analysis of Eight Elements in Cod by Laser Induced Breakdown Spectroscopy

应用激光诱导击穿光谱(laser induced breakdown spectroscopy,LIBS)技术结合单变量定标法、内定标法和偏最小二乘(partial least square,PLS)法对鳕鱼中的P、Fe、Al、Mn、K、Mg、Ca、Na共8种元素进行同时定量分析。制备鳕鱼标准系列,采用电感耦合等离子体发射光谱提供各元素含量的参考值。对鱼肉样品进行压片处理,采集其LIBS光谱,选取各元素特征谱线,并对单变量定标、内定标与PLS 3种定量方法进行比较。结果表明,PLS法优于传统的单变量定标法和内定标法,具有较高的预测准确度和精度,对各元素预测含量的相对误差范围在0.96%~13.27%之间,相对标准偏差范围在2.02%~7.55%之间。结果表明LIBS技术在鳕鱼中多种元素的快速、非定向检测方面具有很大的应用潜力,并可以推广至水产品的快速检测分析,同时为今后开发便携式LIBS水产品检测仪器提供理论依据。

In this study, laser induced breakdown spectroscopy(LIBS) combined with univariate calibration, internal calibration and partial least square(PLS) was used for simultaneous quantitative analysis of P, Fe, Al, Mn, K, Mg, Ca and Na in cod samples. A series of cod standard samples with different concentrations of these elements were prepared, and reference values for each element concentration were determined by inductively coupled plasma atomic emission spectroscopy(ICP-OES). The cod samples were compressed into tablets and their LIBS spectra were collected. The characteristic spectral lines were selected for each element, and the three quantitative methods of univariate calibration, internal calibration and PLS were compared. The results showed that PLS was superior to traditional univariate calibration and internal calibration, and had higher prediction accuracy and precision. The relative error(RE) of the predicted concentration ranged from 0.96% to 13.27%, and the relative standard deviation(RSD) ranged from 2.02% to 7.55%. This work showed that LIBS had great potential in the rapid and non-directional detection of various elements in cod and even other aquatic products, and could lay a foundation for the development of a portable LIBS device for multi-element analysis of aquatic products in the future.