芽菜中高效降解亚硝酸盐菌株的分离鉴定

Isolation and Identification of High-efficiency Nitrite-degrading Bacteria in Sprouts

以芽菜为研究对象,筛选亚硝酸盐高效降解菌,采用分离纯化、分子生物学鉴定等方法对菌株进行分离鉴定。结果表明:通过显色反应、降解能力测定和形态学特征比较,最终筛选出7株具有强降解能力的亚硝酸盐还原菌株,其中A5、A7在浓度为400μg/mL NaNO2的液体培养基中培养24h的降解率最快,分别为80.49%、85.03%,在培养72h后降解率接近99%,进行分子生物学鉴定,结果表明:菌株皆为芽孢杆菌属,其中A5为枯草芽孢杆菌(Bacillus subtilis)、A7为贝莱斯芽孢杆菌(Bacillus velezensis)。

Take sprouts as the research object to screen high-efficiency nitrite-degrading bacteria,and the strains are isolated and identified by the methods of isolation,purification and molecular biological identification.The results show that seven nitrite-degrading strains with strong degradation ability are screened out through chromogenic reaction,determination of degradation ability and comparison of morphological characteristics.The degradation rates of A5 and A7 in NaNO 2 liquid media with the concentration of 400μg/mL culturing for 24 h are the fastest,which is 80.49%and 85.03%respectively.The degradation rate is close to 99%after culturing for 72 h.Furthermore,they are identified by molecular biology,the results show that the strains are Bacillus,and A5 is Bacillus subtilis and A7 is Bacillus velezensiss.