橙色荧光蛋白的研究进展及其应用

Advances in the research and applications of orange fluorescent protein

荧光蛋白(Fluorescent protein,FPs)可作为探针用以探究细胞内分子间相互作用,追踪特定代谢物的代谢途径,对活细胞内的各种代谢过程和细胞通路进行详细、准确的描述。目前已有的FPs几乎已经覆盖了从紫外光到远红外光的所有光谱波段,这些FPs借助高分辨率显微技术应用于生命科学的诸多领域,为生物学的发展作出巨大贡献。橙色FPs通常指光谱区间在540–570nm的FPs,近几年来关于橙色FPs的研究进展较快,并且其作为标记蛋白以及荧光共振能量转移技术(Fluorescence resonance energy transfer,FRET)中的荧光受体在生物学及医学领域得到较多的应用。文中综述了近15年橙色FPs领域的相关研究,重点聚焦橙色FPs的发展和应用,为今后橙色FPs的研究提供依据。

Fluorescent proteins can be used as probes to investigate intercellular molecular interactions and trace the pathway of specific metabolites,thus providing a detailed and accurate description of various metabolic processes and cellular pathways in living cells.Nowadays,the existing fluorescent proteins cover almost all spectral bands from ultraviolet to far-red.These fluorescent proteins have been applied in many fields of bioscience with the help of high-resolution microscopy,making great contributions to the development of biology.It is generally agreed that orange fluorescent proteins refer to the fluorescent proteins at the spectral range of 540–570 nm.In recent years,researches on orange fluorescent proteins have made great progress,and they have been widely applied in the field of biology and medicine as reporter protein and fluorescence resonance energy transfer as fluorescent receptor.This paper reviews the studies in the field of orange fluorescent proteins over the last 15 years,with the special focus on the development and application of orange fluorescent proteins to provide the basis for the future studies.