棘孢木霉挥发性次级代谢产物检测及抑菌活性分析

Antagonistic activity of volatile metabolites from Trichoderma asperellum

木霉是一类具有重要生防价值的丝状真菌。文中首先对分离自浙江省绍兴市和广东省佛山市共12株棘孢木霉Trichoderma asperellum进行平板拮抗评价,然后采用顶空固相微萃取气质联用法(HS-SPME-GC-MS)检测拮抗性较好的两株菌的挥发性次级代谢产物。结果表明,棘孢木霉ZJSX5003和GDFS1009菌丝生长迅速,对尖孢镰孢菌Fusariumoxysporum抑制率分别达73%和74%。挥发性次级代谢产物主要是醇类和酮类,其中包含异丁醇、异戊醇、3-甲基-3-丁烯-1-醇、3-羟基-2-丁酮、2,3-丁二醇和6-正戊基-2H-吡喃-2-酮(6-PAP)。进一步通过体外抑菌试验,证实6-PAP具有较好的抑制尖孢镰孢菌的效果,为开发以木霉菌代谢产物如6-PAP为主要成分的生防制剂提供指导。

Trichoderma spp.is a kind of filamentous fungi with important biocontrol value.Twelve strains of Trichoderma spp.were isolated from the soils of different types of crops in Shaoxing,Zhejiang and Foshan,Guangdong.The antagonistic resistance to Fusarium oxysporum was compared by plate confrontation test.The further analysis of volatile secondary metabolites for two strains were carried out using HS-SPME-GC-MS analysis.The results showed that T.asperellum ZJSX5003 and GDFS1009 had fast growth ability,and the inhibition effects on F.oxysporum were 73%and 74%respectively.Six identical volatile metabolites were detected as follows 2-Methyl-1-propanol,3-Methyl-1-butanol,3-Methyl-3-buten-1-ol,Acetyl methyl carbinol,Butane-2,3-diol and 6-n-pentyl-2H-pyran-2-one(6-PAP).Among them,6-PAP was validated to have a higher inhibitory effect on F.oxysporum in vitro.This study will provide basis for the development of biocontrol agents with metabolites of Trichoderma,such as 6-PAP.