摘要
木霉是一类具有重要生防价值的丝状真菌。文中首先对分离自浙江省绍兴市和广东省佛山市共12株棘孢木霉Trichoderma asperellum进行平板拮抗评价,然后采用顶空固相微萃取气质联用法(HS-SPME-GC-MS)检测拮抗性较好的两株菌的挥发性次级代谢产物。结果表明,棘孢木霉ZJSX5003和GDFS1009菌丝生长迅速,对尖孢镰孢菌Fusariumoxysporum抑制率分别达73%和74%。挥发性次级代谢产物主要是醇类和酮类,其中包含异丁醇、异戊醇、3-甲基-3-丁烯-1-醇、3-羟基-2-丁酮、2,3-丁二醇和6-正戊基-2H-吡喃-2-酮(6-PAP)。进一步通过体外抑菌试验,证实6-PAP具有较好的抑制尖孢镰孢菌的效果,为开发以木霉菌代谢产物如6-PAP为主要成分的生防制剂提供指导。
Trichoderma spp.is a kind of filamentous fungi with important biocontrol value.Twelve strains of Trichoderma spp.were isolated from the soils of different types of crops in Shaoxing,Zhejiang and Foshan,Guangdong.The antagonistic resistance to Fusarium oxysporum was compared by plate confrontation test.The further analysis of volatile secondary metabolites for two strains were carried out using HS-SPME-GC-MS analysis.The results showed that T.asperellum ZJSX5003 and GDFS1009 had fast growth ability,and the inhibition effects on F.oxysporum were 73%and 74%respectively.Six identical volatile metabolites were detected as follows 2-Methyl-1-propanol,3-Methyl-1-butanol,3-Methyl-3-buten-1-ol,Acetyl methyl carbinol,Butane-2,3-diol and 6-n-pentyl-2H-pyran-2-one(6-PAP).Among them,6-PAP was validated to have a higher inhibitory effect on F.oxysporum in vitro.This study will provide basis for the development of biocontrol agents with metabolites of Trichoderma,such as 6-PAP.
作者
陶玲芸
张怡雯
李雅乾
罗来鹏
张曾鲁
陈捷
Lingyun Tao;Yiwen Zhang;Yaqian Li;Laipeng Luo;Zenglu Zhang;Jie Chen(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Key Laboratory of Urban Agriculture(South),Ministry of Agriculture,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2020年第6期1181-1189,共9页
Chinese Journal of Biotechnology
基金
国家重点研发计划(No.2017YFD0200901)
国家自然科学基金(No.31772211)
上海交通大学第十九期创新实践计划(No.IPP19110)资助。
关键词
棘孢木霉
挥发性代谢产物
尖孢镰孢菌
拮抗性
顶空固相微萃取
气质联用
Trichoderma.asperellum
volatile secondary metabolites
Fusarium oxysporum
antagonistic
headspace solid phase microextraction(HS-SPME)
gas chromatography-mass spectrometer(GC-MS)