含醋酸菌粉末的食品对ICR小鼠的酒精性肝损伤的影响

Effect of acetic acid bacterial powder-containing food on acute alcoholicliver injury in ICR mice

探究了含醋酸菌粉末的食品(acetic acid bacterial powder-containing food,AF)对急性酒精性肝损伤的缓解作用。利用美国癌症研究所(Institute of Cancer Research,ICR)小鼠(雄性,20~22 g),随机分为对照组、乙醇组和乙醇+AF组。乙醇组按12 mL/kg口服给予50%(体积分数)乙醇。乙醇+AF组中按小鼠体重分别进行低、中、高3种不同剂量的AF灌胃处理(0.033、0.067、0.200 g/kg)。通过测量肝损伤的血清标志物、氧化应激标志物、甘油三酸酯(triglyceride,TG),并进行肝组织病理学观察,从而比较不同浓度AF对肝脏损伤的缓解作用。结果表明,与对照组相比,通过乙醇摄入可以观察到血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)水平升高和肝脏还原型谷胱甘肽(reduced glutathione,GSH)水平降低。通过摄入0.200 mg/kg的AF可以缓解相应指标的变化。摄入乙醇引起肝脏TG水平升高和组织病理学评分降低,而AF给药可缓解肝脏TG水平升高和组织病理学评分降低。结果表明,摄入AF可减轻急性酒精性肝损伤。

In this study,the effect of ingestion of acetic acid bacterial powder-containing food(AF)on acute alcoholic liver injury was evaluated.ICR mice(male,20-22 g)were randomly divided into control group,ethanol group,and ethanol+AF group.12 mL/kg of 50%ethanol was orally administered to the ethanol group.In ethanol+AF groups,three different doses of AF were administered(0.033,0.067,0.200 g/kg).Serum marker of liver injury and liver marker of oxidative stress were measured.Lipid accumulation in liver was evaluated by quantifying triglyceride and histopathological analysis.Our results showed that increase of serum alanine aminotransferase level and decrease of liver reduced glutathione were observed by ethanol ingestion comparing with control group.These impairments were ameliorated by AF ingestion at dose of 0.200 g/kg.Elevation of liver TG level and histopathological score were induced by ethanol ingestion and alleviated by AF administration.These results suggested that ingestion of AF might ameliorate acute alcoholic liver injury.