Microbio方法与平板计数方法在菌落计数中的比较

A comparative study between Microbio method and plate counting methodon colony counting

为比较Microbio全自动菌落计数方法与普通平板计数方法在菌落计数方面的差异,选取10种菌,按照高中低浓度模拟添加至食品样品中,采用GB 4789.2—2016和GB 4789.15—2016中的方法对样品进行菌落计数。分别从数据准确性、定量限检测、检出时间、人员操作、不同培养基方面进行对比,并将得到的数据进行统计学分析。在数据准确性、人员操作和不同培养基方面,2种方法的回收率在70%以上,相对标准偏差≤0.35。定量限≥5 CFU/mL时,回收率≥70%,相对标准偏差≤0.35。结果表明:Microbio全自动菌落计数方法与普通平板计数法在统计学上并无明显差异,定量限可以达到5 CFU/mL,但在检出时间方面,全自动菌落计数方法优于普通平板法。

In order to compare the difference between the automatic colony counting(Microbio)method and the ordinary plate counting method,ten bacteria strains were added to food samples at high,medium and low concentration.And the standard methods of GB 4789.2—2016 and GB 4789.15—2016 were used for colony counting as control.Data accuracy,quantitative limit,the detection time,personnel operation error and different media were compared and analyzed.In terms of data accuracy,personnel operation error and different media,the recovery rate of the two methods were all over 70%and the relative standard deviation(RSD)≤0.35.When the detection limit≥5 CFU/mL,the recovery rate≥70%and the RSD≤0.35 respectively.The results showed that no significant difference was found between the automatic colony count method(Microbio)and the ordinary plate count method and the quantitative limit could reach 5 CFU/mL.But in terms of the detection time,the automatic colony count method(Microbio)was superior to the ordinary plate method.