基于UHPLC-Q-TOF-MS/MS技术鉴定藤梨根的化学成分

Identification of Chemical Components in Actinidia chinensis Root by UHPLC-Q-TOF-MS/MS

目的:快速鉴定藤梨根的化学成分,为该药材后续的物质基础和质量控制等研究提供参考。方法:运用超高效液相色谱-四极杆-飞行时间串联质谱技术(UHPLC-Q-TOF-MS/MS)进行藤梨根的化学成分检测。色谱柱为Waters XSelect HSS T3,流动相为0.1%甲酸乙腈溶液-0.1%甲酸水溶液(梯度洗脱),柱温为40℃,流速为0.3 mL/min,进样量为3μL;采用电喷雾离子源,以负离子模式采集数据,扫描范围为m/z 50~1500,干燥气温度为350℃,雾化气压力为45 psi,毛细管电压为3500 V,鞘气温度为350℃。根据准分子离子和二级碎片离子信息,结合相关文献以及对照品的保留时间和质谱裂解规律对检出的化学成分进行结构鉴定。结果与结论:共鉴定出58个化学成分,包括16个五环三萜类化合物(如羟基积雪草酸、积雪草酸、山楂酸、科罗索酸、齐墩果酸、熊果酸等)、12个黄酮类化合物(如芦丁、槲皮苷、木犀草苷、紫云英苷等)、17个有机酸类化合物(如隐绿原酸、绿原酸、异绿原酸A、异绿原酸C等)等,有9个化合物(如原花青素B1、B2,木犀草素等)为首次从藤梨根中鉴定出来。UHPLC-Q-TOF-MS/MS技术可快速鉴定藤梨根中的化学成分。

OBJECTIVE:To identify chemical components of Actinidia chinensis root rapidly,and to provide reference for further material basis and quality control study of the crude medicine.METHODS:UHPLC-Q-TOF-MS/MS technique was used to detect chemical components of A.chinensis root.The separation was performed on Waters XSelect HSS T3 column with mobile phase consisted of 0.1%formic acid acetonitrile solution-0.1%formic acid water solution(gradient elution)at the flow rate of 0.3 mL/min.The column temperature was set at 40℃,and sample size was 3μL.Electrospray ion source was adopted,the data was collected under negative ion mode;the scanning range was m/z 50-1500;the drying gas temperature was 350℃,the atomizing air pressure was 45 psi,the capillary voltage was 3500 V,and sheath gas temperature was 350℃.According to the information of excimer ion and secondary fragment ion,the chemical components were identified by combining with the relevant literature,the retention time of the reference substance and the law of mass spectrometry cracking.RESULTS&CONCLUSIONS:Totally 58 chemical components was identified,which included 16 pentacyclic triterpenes(such as hydroxyasiatic acid,asiatic acid,maslinic acid,corosolic acid,oleanic acid,ursolic acid,etc.),12 flavonoids(such as rutin,quercitrin,cynaroside,astragalin,etc.),17 organic acids(such as cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid A,isochlorogenicacid C,etc.).There were 9 components(such as procydanidin B1,B2 and luteolin,etc.)identified for the first time in A.chinensis root.UHPLC-Q-TOF-MS/MS technique can be used for the rapid identification of chemical components in A.chinensis root.