外阻对同步脱氮除硫燃料电池古菌群落的影响

Effects of external resistance on archaeal community in MFCs with simultaneous desulfurization and denitrification

为了研究外阻对同步脱氮除硫燃料电池中古菌群落的影响,构建了4个相同构型的双室微生物燃料电池,分别在100、1 000、10 000Ω和开路状态下运行,并通过高通量测序对污泥古菌群落进行分析。不论外阻如何改变,该系统均表现出高基质去除性能;输出电压和电流密度均随着外阻增大而增大,但电流密度增长幅度较缓。多样性指数及主成分分析结果表明,改变外阻会对阳极室内微生物群落有所影响;而Pearson相关性分析显示,多样性指数与产生电能及环境条件之间并无显著性关系(p>0.05)。结果表明,改变外阻并未对同步脱氮除硫燃料电池中古菌群落丰富度和多样性产生显著性影响。

In order to investigate external resistance on archaeal community in microbial fuel cells(MFCs)with simultaneous desulfurization and denitrification,four MFCs of similar configuration were employed and operated under external resistance of 100 Ω,1 000 Ω,10 000 Ω and open-circuit condition,respectively.Taxonomic distribution of archaeal community in suspension sludge was presented by high-throughput sequencing.All the MFCs show good capacity of substrate removal regardless of external resistance,and the profile of output voltage and current density increase with the increase of external resistance.However,the increase of current density is not as obvious as that of output voltage.The results of diversity indices and principal component analysis suggest that microbial community is affected by external resistance.However,based on Pearson correlation analysis,there is no strong correlation between diversity indices and the electricity generation as well as environmental condition(p>0.05).The results indicate that external resistance does not significantly affect the richness and diversity of archaeal communities in MFCs with simultaneous desulfurization and denitrification.