包头地区仔猪源致泻性大肠杆菌进化分群、生物被膜形成能力及耐药性检测

Evolutionary Cluster and Biofilm Formation Ability of Laxative E. coli from Piglets in Baotou Area and Detection of Drug Resistance

为分析包头地区仔猪源致泻性大肠杆菌进化分群情况、生物被膜形成能力(BF)及耐药性情况。试验采用PCR法、结晶紫微孔板法和K-B药敏纸片法对48株仔猪源致泻性大肠杆菌进行进化分群、生物被膜形成能力及耐药性检测。结果显示,48株仔猪源致泻性大肠杆菌中以B2群和D群流行为主,分别占分离菌株的35.4%和25.0%;48株仔猪源致泻性大肠杆菌对氨苄西林、阿莫西林、新霉素等7种药物耐药率在58.3%以上,对其他药物的耐药率在11.9%~31.3%;48株仔猪源致泻性大肠杆菌中强形成膜能力菌株有20株、中形成膜能力菌株有16株、弱形成膜能力菌株有7株、不形成BF菌株的有5株,分别占分离菌株的41.7%、33.3%、14.6%、10.4%。经分析,48株仔猪源致泻性大肠杆菌的BF形成能力与多黏菌素、新霉素、磺胺间甲氧嘧啶、氟苯尼考、恩诺沙星、氨苄西林、阿莫西林7种药物耐药性存在一定的相关性。本试验为临床中合理用药及该病的防控提供科学依据。

In order to analyze the evolutionary clustering, biofilm forming ability and drug resistance of laxative E.coli from piglets in Baotou area. A total of 48 strains of laxative E.coli from piglets were detected by PCR, crystal violet microplate and K-B susceptibility paper. The results showed that the prevalence of group B2 and group D in 48 strains of laxative E. coli from piglets accounted for 35.4% and 25.0% of the isolates, respectively. The resistance rate of 48 strains of laxative E.coli from piglets to ampicillin, amoxicillin and neomycin was over 58.3%, and to other drugs was between 11.9% and 31.3%. There were 20 strains of strong membrane forming ability in porcine enterodiarrheal E.coli, 16 strains forming medium membrane capacity, 7 strains forming weak membrane forming capacity, and 5 strains without BF, accounting for 41.7%, 33.3%, 14.6% and 10.4% of isolates, respectively. The BF formation ability of 48 strains of laxative E. coli from piglets was correlated with the drug resistance of polymyxin, neomycin, sulfamethoxacil, florfenicol, enrofloxacin, ampicillin and amoxicillin. This study provides scientific basis for rational drug use and prevention and control of the disease in clinic.