摘要
目的探讨miR-125在不同孕期生殖道B族溶血性链球菌(GBS)感染中的表达,并分析其与母婴不良结局的关系。方法选取2015年6月至2018年6月妊娠全程在重庆市开州区人民医院就诊且存在GBS生殖道感染的120例孕妇作为研究对象。根据首次确诊GBS生殖道感染的时间不同分为孕早期组(n=30)、孕中期组(n=43)和孕晚期组(n=47)。采用实时荧光定量(qRT-PCR)检测各组血清miR-125水平;收集三组GBS感染孕妇妊娠不良结局和新生儿不良结局,并分析母婴不良结局与血清miR-125的关系。结果GBS感染孕妇和GBS非感染孕妇血清miR-125比较,差异具有统计学意义(P<0.05);绘制ROC曲线发现,血清miR-125对孕妇GBS生殖道感染具有一定诊断价值(AUC:0.768,95%CI:0.72~0.856,P=0.015,截断值:1.21,灵敏度和特异度分别为78.34%和82.63%)。血清miR-125在孕早期组、孕中期组和孕晚期组依次降低,差异具有统计学意义(P<0.05)。各组GBS生殖道感染孕妇妊娠不良结局比较,差异具有统计学意义(P<0.05),总发生率由低到高依次为孕早期组、孕中期组和孕晚期组。各组GBS生殖道感染孕妇新生儿不良结局比较,差异具有统计学意义(P<0.05),总发生率由高到低依次为孕早期组、孕中期组和孕晚期组。经Pearson相关分析法得知,血清miR-125与GBS生殖道感染孕妇妊娠不良结局相关性不明显差异无统计学意义(P>0.05),但与GBS生殖道感染孕妇新生儿不良结局呈明显正相关差异具有统计学意义(P<0.05)。结论血清miR-125在生殖道GBS感染孕妇中明显高表达,有望成为该病的有效诊断学指标,且与孕期及母婴不良结局密切相关。
Objective To investigate the expression of microRNA-125(miR-125)in genital tract group B hemolytic streptococcus(GBS)infection during different pregnancy stages,and to analyze the relationship between miR-125 and adverse outcomes of mothers and infants.Methods 120 pregnant women with GBS genital tract infection who were treated in Kaizhou District People's Hospital from June 2015 to June 2018 were selected as the study subjects.According to the time of first diagnosis of GBS genital tract infection,they were divided into early pregnancy group(n=30),mid-pregnancy group(n=43)and late pregnancy group(n=47),and 100 normal pregnant women matched with GBS during the same period were selected as the control group.Real-time fluorescence quantitative analysis(qRT-PCR)was used to detect the serum levels of miR-125 in each group.The adverse pregnancy outcomes and neonatal outcomes of GBS infected pregnant women in each group were collected,and the relationship between adverse maternal and neonatal outcomes and serum miR-125 was analyzed.Results There was a significant difference in serum miR-125 between GBS infected pregnant women and non-infected pregnant women(P<0.05).The receiver operating characteristic(ROC)curve showed that serum miR-125 had certain diagnostic value for GBS genital tract infection in pregnant women(AUC:0.768,95%CI:0.722~0.856,P=0.015,truncation value:1.21,sensitivity and specificity of 78.34%and 82.63%,respectively).Serum miR-125 levels decreased in the early pregnancy group,the mid-pregnancy group and the late pregnancy group,with statistically significant differences(P<0.05).There was a significant difference in the adverse pregnancy outcomes of GBS reproductive tract infections among the groups(P<0.05).The total incidence of GBS reproductive tract infections ranged from low to high in the early pregnancy group,the mid-pregnancy group and the late pregnancy group.There was a significant difference in the adverse outcomes of GBS reproductive tract infections among the three groups(P<0.05).The total incidence of GBS was the highest in the early pregnancy group,and lowest in the late pregnancy group.The Pearson correlation analysis showed that serum miR-125 was not significantly correlated with adverse pregnancy outcomes of GBS pregnant women with reproductive tract infection(P>0.05),but positively correlated with the adverse neonatal outcomes(P<0.05).Conclusions Serum miR-125 is highly expressed in pregnant women with GBS infection in reproductive tract,which is expected to be an effective diagnostic indicator,and is closely related to pregnancy and adverse outcomes of mothers and infants.
作者
郑刘芳
刘清秀
李娟
ZHENG Liufang;LIU Qingxiu;LI Juan(Department of Obstetrics,Kaizhou District People's Hospital,Chongqing 405425,China;Department of Obstetrics,Chengdu Women's and Children's Central Hospital,Chengdu 610091,Sichuan,China)
出处
《中国性科学》
2020年第7期73-76,共4页
Chinese Journal of Human Sexuality
关键词
孕期生殖道感染
B族溶血性链球菌
miR-125
母婴不良结局
Reproductive tract infection during pregnancy
Group B hemolytic streptococcus(GBS)
MicroRNA-125(miR-125)
Adverse outcomes of mothers and infants