对掌叶大黄水根提取物体外抗菌活性的初步研究

Preliminary Study on In-vitro Antibacterial Activity of the Extract from Water Root of Zhangye Dahuang

目的:考察甘肃礼县掌叶大黄水根提取物的抗菌活性。方法:采用标准琼脂扩散法,测定抑菌圈直径。结果:掌叶大黄水根乙醇提取物对金黄色葡萄球菌、大肠埃希菌、粪肠球菌及铜绿假单胞菌具有不同程度的抑菌活性,对大肠埃希菌抑制作用最强[抑菌圈直径为(15.5±0.4)mm],对金黄色葡萄球菌和粪肠球菌抑制作用相当[抑菌圈直径分别为(14.8±0.8)mm和(14.5±0.4)mm)],对铜绿假单胞菌抑制作用略弱[抑菌圈直径为(11.8±0.2)mm]。结论:掌叶大黄水根乙醇提取物对细菌有一定的抗菌活性,对临床分离的真菌(白色念珠菌)则无明显抑菌活性。

Objective:To investigate antibacterial activity of the extract from water root of Zhangye Dahuang(Rheum palmatum L.).Methods:Standard agar diffusion method was used to detect the diameter of inhibition zone.Results:Ethanol extract from water root of Zhangye Dahuang possesses different degrees of antibacterial effects on Staphylococcus aureus(SA),E.coli,Enterococcus faecalis and Pseudomonas aeruginosa,it owns the strongest inhibitory action on E.coli[the diameter of inhibition zone was(15.5±0.4)mm],the same inhibitory action on SA and Enterococcus faecalis[the diameters of inhibition zone were(14.8±0.8)mm and(14.5±0.4)mm],and weaker inhibitory action on Pseudomonas aeruginosa[the diameter of inhibition zone was(11.8±0.2)mm].Conclusion:Ethanol extract from water root of Zhangye Dahuang shows certain antibacterial effects on the bacteria,while no obvious antibacterial activity on fungi(Candida albicans)separated from clinic.