醉鱼草对鲫麻醉效果及血清生化指标的影响

Effects of Buddleja lindleyana Fortune on anaesthesia and serum biochemical indices in crucian carp Carassius auratus

为探索新型鱼用麻醉剂的麻醉效果,采用不同浓度(2、4、6 g/L)醉鱼草Buddleja lindleyana Fortune粗提物水溶液对平均体质量为220 g左右的鲫Carassius auratus进行麻醉效果试验,并分析了浓度为6 g/L的醉鱼草水溶液对鲫血清生化指标的影响。结果表明:随着醉鱼草粗提物水溶液浓度的提升,鲫麻醉所需时间减少,复苏所需时间增加,呼吸频率下降;3个浓度条件下鲫复苏率均为100%,其中浓度为6 g/L的醉鱼草水溶液对鲫麻醉效果最为明显;对鲫血清生化指标检测表明,醉鱼草粗提物水溶液对鲫有麻醉作用,CAT的显著升高也表明其激发了肝细胞的氧化防御反应,细胞通过提高CAT的生产速率来抵御物质带来的氧化损伤;安全性评价结果显示,醉鱼草对鲫无毒性。研究表明,醉鱼草对鲫有明显的麻醉效果,可作为新型鱼用麻醉剂进行开发与使用。

Crucian carp Carassius auratus with body weight of about 220 g was held in a 50 L tank and exposed to crude extract of plant Buddleja lindleyana Fortune at a dose of 2,4,and 6 g/L in order to explore the anaesthetic effect of a new type of fish anaesthetic.Activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),acid phosphatase(ALP),lactate dehydrogenase(LDH-L),catalase(CAT),and superoxide dismutase(SOD)and cortisol content(COR)were determined in blood of caudal vein of crucian carp sampled by tail cutting before anesthesia(control group),just unbalanced,recovery for 0 h,and recovery for 12 h.The results showed that crucian carp was anesthetized in shortened time,recovery in longe time and had low respiratory rate with the increase in the concentration of the crude extract.The crucian carp had recovery rate of 100%within the three concentrations of the crude extract,with the most obvious anesthetic effect at crude extract concentration of 6 g/L.The blood biochemical detection revealed that there was anesthetic effect of the crude extract of Buddleja lindleyana Fortune(6 g/L)on crucian carp,with significant increase in CAT activity,indicating that the crude extract stimulated the oxidative defense reaction of hepatocytes which resisted the oxidative damage caused by substances by increasing the production rate of CAT.The findings showed that the aqueous solution had obvious anesthetic effect on crucian carp and was used as a new type of fish anesthetic.