Caveolin-1在内毒素性肺损伤所致的肺纤维化中的表达及临床意义

Caveolin-1 Expression and Clinical Significance in Pulmonary Fibrosis Caused by Endotoxic Lung Injury

目的探讨小窝蛋白-1(Caveolin-1)在内毒素(LPS)性肺损伤所致的肺纤维化中的表达及临床意义。方法选择健康雄性SD成年大鼠60只,随机分成对照组、模型组和干预组,各20只。对照组尾静脉注射生理盐水2 ml,模型组、干预组均采用LPS 5 mg/kg以生理盐水稀释至2 ml尾静脉注射,诱导肺损伤纤维化模型,干预组在给予诱导前经尾静脉先给予TGF-β1抗体2μg/100 g,给药后第1、5、14、21天分别处死每组SD大鼠5只,取大鼠肺组织行HE染色观察病理变化,免疫组化方法检测肺组织内的肺组织胶原蛋白活性(Hpy)、Ⅰ型转移生长因子受体(TβRⅠ)以及Caveolin-1的表达情况。结果①对照组肺泡结构基本正常,肺泡腔内无明显渗出及炎症细胞浸润;模型组第1天以急性肺泡炎症为主,第5天时肺泡炎症较前有所减轻,第14天时开始出现肺纤维化,第21天时出现广泛肺纤维化;干预组病理改变与模型组呈同一规律,但肺泡炎症和肺纤维化程度轻于模型组;②给药后第1、5、14、21天,模型组和干预组Hpy表达均高于对照组,干预组第5、9、14天低于模型组,差异有统计学意义(P<0.05);模型组与干预组肺组织TβRⅠ表达均高于对照组,模型组第14、21天高于干预组,差异有统计学意义(P<0.05);模型组与干预组血清Caveolin-1水平低于对照组,且干预组高于模型组,差异有统计学意义(P<0.05)。结论Caveolin-1可减轻内毒素性肺损伤的程度,并能抑制纤维化过程,具有一定的肺保护作用。

Objective To investigate the expression and clinical significance of Caveolin-1 in pulmonary fibrosis caused by endotoxin(LPS)lung injury.Methods 60 healthy male male SD rats were randomly divided into control group,model group and intervention group,20 rats each.The control group was injected with 2 ml of normal saline.The model group and the intervention group were injected with LPS 5 mg/kg diluted with normal saline to 2 ml of the tail vein to induce the lung injury fibrosis model.The intervention group passed the tail vein before induction.TGF-β1 antibody was given 2μg/100 g,and 5 SD rats in each group were killed on days 1,5,14 and 21 after administration.The lung tissues of the rats were taken for HE staining to observe pathological changes and detected by immunohistochemistry Lung tissue collagen activity(Hpy),type Ⅰ transfer growth factor receptor TβRⅠand Caveolin-1 expression in lung tissue.Results①The alveolar structure of the control group was basically normal,and there was no obvious exudation and infiltration of inflammatory cells in the alveolar cavity;the model group was dominated by acute alveolar inflammation on the first day,and the alveolar inflammation was lessened on the fifth day than before,and pulmonary fibers began to appear on the 14th day pulmonary fibrosis occurred on the 21st day;the pathological changes of the intervention group were the same as the model group,but the degree of alveolar inflammation and pulmonary fibrosis was lighter than that of the model group;②On days 1,5,14,and 21 after administration,the Hpy expression of the model group and the intervention group was higher than that of the control group,and the intervention group was lower than that of the model group on days 5,9,and 14,the difference was statistically significant(P<0.05);the lung tissue TβRⅠ expression of the model group and the intervention group was higher than that of the control group,and the model group was higher than that of the intervention group on days 14 and 21,the difference was statistically significant(P<0.05);Serum Caveolin-1 levels in the model group and the intervention group were lower than the control group,and the intervention group was higher than the model group,the difference was statistically significant(P<0.05).Conclusion Caveolin-1 can reduce the degree of endotoxin-induced lung injury,and can inhibit the fibrosis process,and has a certain lung protective effect.