干化学法与尿沉渣分析仪法检测儿童尿液标本的结果比较

Comparison Between the Results of Dry Chemistry and Urine Sediment Analyzer in Detecting Urine Samples of Children

目的比较尿液干化学法与尿沉渣分析仪法对儿童尿液标本中红细胞和白细胞的检测结果的影响。方法收集成都市妇女儿童中心医院2019年1~12月住院及门诊就诊的10000例儿童新鲜尿液标本,采用尿液干化学分析仪法、尿沉渣全自动分析仪法及尿沉渣显微镜检法进行检测,并以显微镜检作为对照,比较三种方法检测儿童尿液红细胞、白细胞的结果。结果全自动尿沉渣分析仪法在检测红细胞时敏感性、特异性均高于尿液干化学分析仪法(92.35%vs 64.73%)、(96.32%vs 95.05%),差异有统计学意义(P<0.05),而尿液干化学分析仪法检测红细胞的假阳性率和假阴性率高于全自动尿沉渣分析仪法(4.95%vs 3.68%)、(35.27%vs 7.65%),差异有统计学意义(P<0.05)。全自动尿沉渣分析仪法在检测白细胞时敏感性高于尿液干化学分析仪法(86.71%vs 36.19%),但是特异性低于尿液干化学分析仪法(94.17%vs 98.22%),差异有统计学意义(P<0.05);尿液干化学分析仪法检测白细胞的假阳性率低于全自动尿沉渣分析仪法(1.78%vs 5.83%),但假阴性率高于全自动尿沉渣分析仪法(63.81%vs 13.29%),差异有统计学意义(P<0.05)。结论全自动尿沉渣分析仪法检测红细胞的敏感性、特异性优于尿液干化学法,尿液干化学分析仪法在检测白细胞的特异性优于全自动尿沉渣分析仪法,需将其检测方法结合使用,才能快速、高效地为临床提供更为准确、可靠的检验数据。

Objective To compare the effect of urine dry chemistry and urine sediment analyzer on the detection results of red blood cells and white blood cells in urine samples of children.Methods Collecting 10,000 fresh urine samples of Chengdu Women's and Children's Central Hospital from January to December 2019 inpatients and outpatients,using UC-3500 urine dry chemical analyzer,UF-5000 automatic urine sediment analyzer and urine sediment microscopic examination was used as a control,and microscopic examination was used as a control to compare the results of three methods for detecting urine red blood cells and white blood cells in children.Results The sensitivity and specificity of the automatic urine sediment analyzer method in detecting red blood cells are higher than that of the urine dry chemical analyzer method(92.35%vs 64.73%)and(96.32%vs 95.05%),the difference was statistically significant(P<0.05),and the false positive rate and false negative rate of erythrocyte detection by urine dry chemical analyzer method are higher than that of automatic urine sediment analyzer method(4.95%vs 3.68%),(35.27%vs 7.65%),the difference was statistically significant(P<0.05).The automatic urine sediment analyzer method is more sensitive than the urine dry chemical analyzer method(86.71%vs 36.19%)in detecting leukocytes,but the specificity is lower than the urine dry chemical analyzer method(94.17%vs 98.22%),the difference was statistically significant(P<0.05);the false positive rate of urine dry chemical analyzer for detecting white blood cells is lower than that of automatic urine sediment analyzer(1.78%vs 5.83%),but the false negative rate is higher than that of automatic urine Slag analyzer method(63.81%vs 13.29%),the difference was statistically significant(P<0.05).Conclusion The sensitivity and specificity of the automatic urine sediment analyzer for detecting red blood cells is better than that of urine dry chemistry.The urine dry chemical analyzer is better than the automatic urine sediment analyzer for detecting white blood cells.The combined use of detection methods can provide more accurate and reliable test data for clinic quickly and efficiently.