兰坪虫草多糖组分分析及免疫活性研究

Studies on the component analysis and immune activities ofOphiocordyceps lanpingensis polysaccharides

从兰坪虫草中提取多糖,对其分子量、单糖组成和官能团特征进行分析,并探讨兰坪虫草多糖对免疫力的影响。结果表明,兰坪虫草多糖分子量为3.2×10~5 Da,主要由甘露糖、半乳糖和葡萄糖组成,其摩尔百分比为13.38∶5.31∶81.31,可能为含有糖醛酸和吡喃环的酸性多糖。兰坪虫草多糖可显著提高免疫抑制小鼠的免疫器官指数,减少免疫抑制小鼠脾脏组织红髓区域充血,改善碳粒廓清能力,提高免疫抑制小鼠单核-巨噬细胞的吞噬功能,促进抗绵羊红细胞抗体的生成,促使ConA诱导的T淋巴细胞增殖,提高免疫相关细胞因子IL-2和IL-10的基因表达水平,说明兰坪虫草多糖具有增强小鼠免疫力的作用。

The polysaccharides was extracted from O.lanpingens,and the molecular weight,monosaccharide composition and functional group characteristics were analyzed.Furthermore,the effect of O.lanpingensis polysaccharides on immunity was investigated.The results showed that OLP had a molecular weight of 3.2×10~5 Da,and it was mainly composed of mannose,galactose and glucose with a molar ratio of 13.38∶5.31∶81.31.OLP might be an acid polysaccharide containing uronic acid and pyran ring.OLP could significantly increase the spleen index,reduce red pulp area congestion of spleen,improve the ability of clearing carbon particles,strengthen phagocytic function of mononuclear phagocytic system,promote the production of antibodies against sheep red blood cells and increase ConA-induced T lymphocyte proliferation.Moreover,OLP also increased gene expression levels of immune-related cytokines IL-2 and IL-10.These studies indicated that OLP could enhance the immunity of mice.