间皮素与人附睾蛋白4在上皮性卵巢癌中的意义和诊断价值

The Significance and Diagnostic Value of Mesothelin and Human Epididymis Protein 4 in Epithelial Ovarian Cancer

目的探究间皮素与人附睾蛋白4(human epididymis protein 4,HE4)在上皮性卵巢癌(epithelial ovarian cancer,EOC)中的意义和诊断价值。方法随机选择2018年5月至2019年5月间的EOC患者90例作为EOC组,收集同期卵巢囊肿患者50例作为对照组,通过问卷调查的方式收集基本资料,经过手术或全身PET-CT检测EOC转移情况。通过免疫组化染色检测组织中间皮素的表达水平,分别通过酶联免疫吸附法和电化学发光分别检测血清中间皮素和HE4水平。结果免疫组化研究结果显示间皮素主要表达于细胞质。EOC组织中间皮素的阳性率和血清中间皮素的水平均显著高于对照组(P<0.05)。血清中HE4也显著高于对照组(P<0.05)。组织和血清中间皮素(r=0.634,P<0.05)呈现正相关关系。是否绝经、肿瘤大小对EOC组织中间皮素、血清中间皮素和HE4水平的影响不大(P>0.05)。FIGO分期为III/IV期、2/3级T分期、1级N和1级M分期的EOC组织中间皮素、血清中间皮素和HE4水平显著升高(P<0.05)。结论组织和血清中间皮素、血清中HE4在EOC中均过表达,并且高水平的间皮素和HE4与EOC的不良表型和转移风险有关,可能作为EOC的生物标志因子。

Objective To explore the significance and diagnostic value of mesothelin and human epididymis protein 4(HE4)in epithelial ovarian cancer(EOC).Methods 90 patients with EOC from May,2018 to May,2019 were randomly selected as the EOC group,while 50 cases of ovarian cyst were collected as a control group.We then collected basic information of these patients through questionnaires.EOC metastasis status was detected by surgery or whole-body PET-CT.The expression level of tissue mesothelin was examined by immunohistochemical staining.Serum intermediate mesothelin and HE4 levels were measured by enzyme-linked immunosorbent assay and electrochemiluminescence,respectively.Results The results of immunohistochemical studies showed that mesothelin was mainly expressed in the cytoplasm.The positive rate of mesothelin in EOC tissues and the level of serum mesothelin in the EOC group were significantly higher than those in the control group(P<0.05).HE4 in serum in the EOC group was also significantly higher than that in the control group(P<0.05).There was a positive correlation between tissue and serum mesothelin(r=0.634,P<0.05).Whether menopause and tumor size had little effect on EOC tissue mesothelin,serum mesothelin and HE4 levels(P>0.05).The levels of EOC tissue mesothelin,serum mesothelin,and HE4 in FIGO stage III/IV stage,2/3 grade T stage,grade 1 N and grade 1 M stages were all significantly increased(P<0.05).Conclusion Tissue and serum mesothelin and serum HE4 are overexpressed in EOC,and high levels of mesothelin and HE4 are associated with a poor phenotype and the risk of metastasis of EOC,which could possibly serve as biomarkers of EOC.