sprouty4蛋白对角质形成细胞增殖及分化的影响

Effects of sprouty4 protein on keratinocyte proliferation and differentiation

目的:探证sprouty4(SPRY4)蛋白对角质形成细胞增殖及分化的影响。方法:以人永生化表皮细胞株HaCaT细胞作为实验对象,实验组HaCaT细胞采用基因敲降技术进行SPRY4蛋白抑制物的转染,对照组HaCaT细胞不作任何处理。运用实时荧光定量PCR技术(RT-qPCR)检测实验组与对照组中sprouty4蛋白对HaCaT细胞分化指标Involucrin、CK1与CK10的影响,运用CCK-8实验检测HaCaT细胞的增殖功能。结果:RT-qPCR结果显示,实验组HaCaT细胞中SPRY4基因敲降成功,敲降率约为94.75%。与对照组相比,实验组HaCaT细胞中分化指标Involucrin、CK1与CK10表达水平降低;CCK-8实验结果显示,与对照组相比,实验组HaCaT细胞增殖能力增强。结论:SPRY4蛋白表达下降对细胞增殖起到促进作用,对细胞分化起到抑制作用。

Objective:To explore the effects of sprouty4(SPRY4) protein on keratinocyte proliferation and differentiation.Methods:Human immortalized keratinocytes(HaCaT) cells were transfected with SPRY4 protein siRNA, and HaCaT cells without transfection served as controls. Real-time fluorescence quantitative PCR(RT-qPCR) was used to assess expression levels of mRNA for HaCaT cells differentiation(Involucrin, CK1 and CK10), and the CCK-8 assay was used to measure HaCaT cell proliferation.Results:RT-qPCR results showed 94.75% reduction in expression levels of SPRY4 mRNA in comparison to the controls. Moreover, knockdown of SPRY4 decreased expression levels of involucrin, CK1 and CK10 mRNA, while stimulating HaCaT cells proliferation.Conclusion:SPRY4 protein stimulates keratinocyte proliferation, while inhibiting differentiation.