大鼠肾脏缺血再灌注过程的光学相干层析成像监测

Monitoring of Ischemia-Reperfusion Process of Rat Kidney Using Optical Coherence Tomography

光学相干层析成像(OCT)具有分辨率高、成像深度深和成像速度快的优点,为评估肾脏状态提供了一种新方法。肾脏缺血再灌注会导致肾功能单元发生损伤,采用无损成像可以实时快速地评价肾脏的活性状态。采用中心波长为1300 nm的SDOCT(spectral-domain OCT)对Wistar大鼠肾脏进行活体二维和三维成像,图像能够清晰地显示肾皮质层的肾小管微结构。结果表明:OCT对肾脏缺血再灌注过程响应灵敏,在肾脏状态评估方面具有广阔的应用前景。

Owing to the advantages of optical coherence tomography(OCT),such as high resolution,deep imaging depth,and fast imaging speed,it facilitates a new method for the evaluation of renal status.The renal ischemia-reperfusion(IR)process can cause damage to renal functional units;such damages can be quickly estimated using noninvasive optical imaging in real time.Herein,using spectral-domain OCT(SDOCT)with 1300-nm center wavelength,two-and three-dimensional images of Wistar rat kidneys were collected in vivo.The obtained images clearly show the microstructure of uriniferous tubules in the renal cortex.Moreover,results demonstrate that OCT imaging is prominent in the evaluation of renal status owing to the sensitivity of OCT to IR process.