EPF单抗联合顺铂对家畜膀胱肿瘤抑瘤效果的影响——基于膀胱肿瘤裸鼠移植瘤模型

Antitumor effect of EPF monoclonal antibody combined with cisplatine on animal bladder cancer in livestock:Based on a bladder tumor model in nude mice

为观察早孕因子单克隆抗体(EPF-McAb)联合顺铂(DDP)对膀胱癌细胞EJ裸鼠移植瘤的抑制作用,常规将EJ细胞接种于裸鼠右侧背部皮下,待肿瘤长至直径约5 mm时随机分为4组,每组5只,即空白对照组、顺铂组、低剂量单抗联合组和高剂量单抗联合组。定期测量裸鼠肿瘤体积,绘制肿瘤生长曲线。给药2周后处死裸鼠,剥取肿瘤,称瘤重,计算抑瘤率。通过苏木精-伊红(Hematoxylin-eosin,HE)染色观察细胞病理学改变;免疫印迹法检测早孕因子(EPF)的表达情况。结果表明,顺铂组、低剂量单抗联合组和高剂量单抗联合组裸鼠移植瘤生长速度均慢于空白对照组。与顺铂组比较,低剂量单抗联合组和高剂量单抗联合组裸鼠末次给药的移植瘤体积分别为(131.05±8.26)和(97.92±7.35)mm^3。低剂量单抗联合组抑瘤率(35.78%)和高剂量单抗联合组抑瘤率(53.04%)均高于顺铂组抑瘤率(22.36%),差异均达显著水平(P<0.05)。与空白对照组比较,单抗联合组裸鼠EPF蛋白的表达量下降(P<0.05)。表明EPF-McAb联合顺铂可抑制膀胱癌细胞EJ在裸鼠体内增殖,其机制可能通过抑制EPF的表达发挥作用。

To investigate the antitumor effects of early-pregnancy-factor monoclonal antibody(EPF-McAb)combined with cisplatin(DDP)on human bladder cancer EJ cell in nude mice,and to explore its possible mechanism,a melanin xenograft model of nude mice was established. When the diameter of the transplanted tumor grew to 5 mm,the mice were randomly divided into 4 groups,a blank group with no treatment,a control group treated with cisplatin,and two treatment group treated with low dosage EPF-McAb combination and high dosage EPF-McAb combination,respectively. The volume of transplanted tumor was measured every 2 days after the administration tp plot the tumor growth curve. On the 10th day after the last administration,the tumors were taken and weighed to calculate the tumor inhibition rate. The pathology changes of carcinoma were observed after HE staining by light microscope. The expression of EPF protein was detected by Western blot. The results showed that the growth rate of the transplanted tumors in the two treat groups were significantly slower than that in the blank group and the control group(P<0.05). The tumor inhibition rate of the transplanted tumors in the low-dose EPF-McAb group and high-dose EPF-McAb was 35.78% and 53.04%,respectively,which were both significantly higher than that in the control group(with tumor inhibition rate of 22.36%)(P<0.05). And the expression of EPF protein in treatment groups was also decreased. It indicated that EPF-McAb combined with DDP can effectively inhibit the proliferation of bladder cancer EJ cells in nude mice. The mechanism may be related to down-regulation of EPF protein expression.