芍黄颗粒对小鼠银屑病模型的治疗作用研究

Therapeutic effect of Shaohuang granules on psoriasis mice

为了探讨芍黄颗粒对小鼠银屑病模型的治疗作用,采用咪喹莫特致小鼠银屑病模型观察芍黄颗粒对银屑病模型小鼠皮损组织病理变化、表皮厚度及皮肤组织中增殖细胞核抗原(PCNA)表达的影响;采用小鼠阴道上皮病理模型及小鼠尾鳞片上皮病理模型观察芍黄颗粒对小鼠阴道上皮有丝分裂及小鼠尾鳞片表皮颗粒层形成的影响。结果表明,与模型组比较,芍黄颗粒各剂量组均能显著减小银屑病模型小鼠皮肤厚度(P<0.05),芍黄颗粒5.0、10.0 g/kg剂量对小鼠背部皮损有改善作用且能显著减少小鼠皮肤组织中PCNA蛋白表达量;芍黄颗粒10.0 g/kg剂量能明显抑制小鼠阴道上皮细胞有丝分裂,对小鼠尾鳞片表皮颗粒层形成有显著促进作用。表明芍黄颗粒可通过影响表皮细胞过度增殖、角化不全而减小皮肤厚度、改善皮损,抑制阴道上皮细胞有丝分裂,促进小鼠尾鳞片表皮颗粒层形成等来治疗银屑病,为银屑病治疗的有效药物。

To evaluate the effects of Shaohuang granules on psoriasis mice,a imiquimod-induced psoriasis model was constructed and used to observe the influence of Shaohuang granules on the pathological changes,epidermal thickness,the expression of PCNA in psoriatic lesions. The effects of Shaohuang granules on the mitosis of vaginal epithelial cells in estrogen period and the granular layer of mice tail epidermis were also observed. The results showed that Shaohuang granules could obviously decreased the skin thickness of psoriasis mice(P<0.05). 10 g/kg and 5 g/kg dose of Shaohuang granules could improve the mice skin lesions and reduce the PCNA expressions significantly. 10 g/kg dose of Shaohuang granules could obviously inhibit the mitosis of mice vaginal epithelium in estrogen period and had distinct promotion effect on the granular layer formation of mice tail epidermis. It indicated that Shaohuang granules are effective on treating psoriasis. It can decrease the skin thickness and improve skin lesion by affecting the epidermal parakeratosis and epidermal over-proliferation. It also can inhibit the mitosis of vaginal epithelial cells and promote the epidermal granules formation of mice tail.