海南东寨港红树林内生真菌Aspergillus fumigatus SAS10吡喃酮类代谢产物

Pyrone metabolites of a mangrove endophytic fungus Aspergillus fumigatus SAS10isolated from Dongzhai Harbor,Hainan

采用大米培养基,对无瓣海桑内生真菌Aspergillus fumigatus SAS10进行静置发酵,运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、以及半制备高效液相等分离手段对发酵产物进行分离纯化,获得7个化合物。经分析质谱、核磁等波谱数据,并通过与文献对比鉴定化合物为:aspergyllone(1),(R)-2-(羟基(苯基)甲基)-4H-吡喃-4-酮(2),carbonarone A(3),campyrone A(4),campyrone C(5),asperfurandione A(6)和dibutylterephthalate(7)。所得7个化合物均为首次报道为烟曲霉次级代谢产物。

The mangrove endophytic fungus Aspergillus fumigatus SAS10 from Sonneratia apetala of Dongzhai haror in Hainan was fermented by using rice medium. The extract of fermentation culture was subjected to column chromatography on silica gel,column chromatography on Sephadex LH-20,and semi-preparative HPLC. Seven compounds were obtained. By analyzing mass and NMR spectroscopic data,their structures were identified as aspergyllone(1),(R)-2-(hydroxy(phenyl)methyl)-4 H-pyran-4-one(2),carbonarone A(3),campyrone A(4),campyrone C(5),asperfurandione A(6)and dibutylterephthalate(7),respectively. All of compounds obtained are reported firstly from secondary metabolites of the mangrove endophytic fungus Aspergillus fumigatus.