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4-甲基伞形酮对瘢痕疙瘩成纤维细胞的抗炎作用及机制研究 被引量:2

The Study on Anti-inflammatory Effect and Mechanisms of 4-methylumbelliferone in Keloid Fibroblasts
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摘要 目的:探讨4-甲基伞形酮(4-methylumbelliferone,4MU)对瘢痕疙瘩成纤维细胞的作用及机制研究。方法:体外培养的原代瘢痕疙瘩成纤维细胞(KFs)分别用浓度为0、0.2、0.4、0.8mM的4MU处理,采用CCK-8法和细胞划痕实验检测4MU对KFs细胞增殖和迁移能力的影响,通过ELISA、qPCR、免疫荧光染色和Western-bloting技术检测4MU对KFs炎症相关因子表达的影响。结果:4MU不仅抑制KFs细胞增殖迁移,而且可减少KFs对IL-6和IL-8的分泌,同时抑制HAS2和HAS3 mRNA的表达及α-SMA、TLR4、CD44、NF-κB1、P65蛋白的合成。结论:4MU可以抑制KFs增殖和迁移,并可通过抑制NF-κB通路相关基因的表达,减少KFs炎症因子分泌,因此,4MU有可能成为治疗瘢痕疙瘩的新药物。 Objective To investigate the effect of 4-methylumbelliferone(4MU)on keloid fibroblasts(KFs).Methods Primary keloid fibroblasts were respectively treated with 4MU at concentrations of 0,0.2,0.4,and 0.8 mM.The proliferation and migration ability of KFs were detected by CCK-8 assay and cell migration assay.ELISA,qPCR,immunofluorescence and Western-bloting techniques were applied to study the effects of 4MU on the expression of inflammation-related genes in KFs.Results 4MU not only inhibited the proliferation and migration of KFs,but also inhibited the secretion of IL-6 and IL-8.Additionally,4MU inhibited the expression of HAS2 and HAS3,and reduced the synthesis ofα-SMA,TLR4,CD44,NF-κB1 and P65 proteins in KFs.Conclusion 4MU treatment can not only inhibit the activity of KFs,but also decrease the secretion of inflammatory by downregulating the expression of NF-κB signal pathway.Therefore,4MU has the potential to be a new drug for the cure of keloids.
作者 牛星堂 林珣珣 朱昭炜 许澍洽 唐庆 NIU Xing-tang;LIN Xun-xun;ZHU Zhao-wei;XU Shu-qia;TANG Qing(Department of Plastic Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,Guangdong,China)
出处 《中国美容医学》 CAS 2020年第7期97-100,共4页 Chinese Journal of Aesthetic Medicine
基金 广东省自然科学基金(编号:2016A030313243)。
关键词 4-甲基伞形酮 瘢痕疙瘩 炎症因子 TLR4 NF-κB1 作用机制 4-methylumbelliferone keloid inflammatory factors TLR4 NF-κB1 mechanism of action
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  • 1M.Mehta,O.A.Branford,K.J.Rolfe.The evidence for natural therapeutics as potential anti-scarring agents in burn-related scarring[J].Burns & Trauma,2016,4(2):78-89. 被引量:6
  • 2刘嘉锋,张一鸣,易传勋,孙家明,李薇薇.性激素受体在瘢痕中的表达及与细胞周期调控蛋白相互关系的研究[J].中华整形外科杂志,2004,20(4):265-267. 被引量:17
  • 3王志军,刘林蟠.美容外科学[M].北京:人民卫生出版社,2012:117-118.
  • 4Lu WS1,Zheng XD,Yao XH,et al. Clinical and epidemiologicalanalysis of keloids in Chinese patients[J].Arch Dermatol Res,2015,307(2):109-114.
  • 5Bashir MM, Ahmad H, Yousaf N, et al. Comparison of single intraoperative versus an intra operative and two post operative injectionsof the triamcinolone after wedge excision of keloids of helix[J].J PakMed Assoc,2015,65(7):737-741.
  • 6Khan MA,Bashir MM,Khan FA. Intralesional triamcinolone aloneand in combination with 5-fluorouracil for the treatment of keloidand hypertrophic scars[J].J Pak Med Assoc,2014,64(9):1003-1007.
  • 7Payapvipapong K1, Niumpradit N, Piriyanand C, et al. Thetreatment of keloids and hypertrophic scars with intralesionalbleomycin in skin of color[J].J Cosmet Dermatol,2015;14(1):83-90.
  • 8Shaarawy E, Hegazy RA,Abdel Hay RM. Intralesional botulinumtoxin type A equally effective and better tolerated than intralesionalsteroid in the treatment of keloids: a randomized controlled trial[J].JCosmet Dermatol,2015,14(2):161-166.
  • 9van Leeuwen MC1, Stokmans SC, Bulstra AE, et al. High-doseratebrachytherapy for the treatment of recalcitrant keloids: a unique,effective treatment protocol[J].Plast Reconstr Sur g,2014,134(3):527-534.
  • 10Song C,Wu HG,Chang H,et al.Adjuvant single-fractionradiotherapy is safe and effective for intractable keloids[J].J RadiatRes,2014,55(5):912-916.

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