AHLs类信号分子对IFFAS工艺影响研究

Research on effects of N-acyl-homoserine-lactones(AHLs)signal molecules on IFFAS technology

通过在传统载体基料(聚乙烯)中添加聚季铵盐-10改善载体的亲水性和亲电性,以促进微生物在载体表面的生长,并通过向反应器中添加N-酰基高丝氨酸内酯(AHLs)类信号分子改良生物膜特性以及微生物结构.实验结果表明,相比于传统载体,改性载体表面生物膜增长速率较快,生物膜生长稳定后载体表面生物膜量较高,且在AHLs作用下改性载体表面生物膜含有较多的蛋白质和多糖.实验运行阶段,投加载体反应器的COD去除效果优于活性污泥反应器;投加改性载体和AHLs的反应器的氨氮和总氮去除效果最优,去除率分别能够达到94.8%和65.3%,且各运行阶段出水水质明显优于其他反应器.微生物群落分析表明,AHLs能够明显提高反应器中与群体感应密切相关的反硝化菌Zoogloea的相对丰度.

The hydrophilicity and electrophilicity of the carrier were enhanced by adding polyquaternium-10 to the conventional carrier material(polyethylene)to promote the growth of microorganisms on the carrier surface.The properties of biofilm and microbial structure were changed by adding N-acyl-homoserine-lactones(AHLs)signal molecules into the reactors.The experimental results show that the growth rate of biofilm on the surface of the modified carriers is higher than that of the conventional carriers,and the attached biomass increases after biofilm growing mature.The protein and polysaccharide contents in the biofilm of the modified carriers under the effects of AHLs can reach a higher level.During the experiment,the COD removal performance of the reactor with carrier is better than that of activated sludge reactor.The ammonium and total nitrogen removal performance of the reactor with modified carrier and AHLs is the best,which can reach to 94.8%and 65.3%respectively.The effluent quality of the reactor with modified carrier and AHLs is higher than that of other reactors throughout the experiment.Besides,the microbial community analysis shows that AHLs can improve the relative abundance of denitrifying bacteria Zoogloea known as a quorum sensing bacteria in the reactor.